Product > Optical Microscopes> Adv Biological Micrsocpes > Confocal LSM Mscope System

Advanced Biological Microscopes

  Modular Confocal Microscope System - CONFOCAL C1Plus

• Optical performance in a class of its own
• FRAP capabilities
• Interchangeable filters
• 3-channel simultaneous detection
• Modular design saves space and facilitates upgrading
  All main components are modular including the laser box, scanner head, and detector module, saving desk space and allowing easy upgrades and maintenance. The scanner head can be easily mounted on either upright or inverted microscopes. 
• Image sizes of up to 2048 by 2048 at 12 bit image depth can be easily scanned.
• FRAP capabilities - ROI scan with an optional Acousto Optical Modulator (AOM)
• Interchangeable filters
• 3-channel simultaneous detection
  The C1 supports almost any imaging technique currently required, including simultaneous 3-channel fluorescence, 3-channel plus DIC, time-lapse recording, and spatial analysis.
• Intuitive software allows all the settings and procedures required to capture live images to be performed in a single window (Tiff-Converter and Acquire-Setting Reproduction programs have also been pre-installed.)
• Crosstalk-free- Images can be captured without crosstalk via sequential scan
• 3D imaging via a simple yet versatile operation menu

1. Decide the upper limit of the specimen.
2. Decide the lower limit of the specimen.
3. Activate the Z image mode.
4. Click to capture.

• Time lapse- Most effective for observing changes of living cells over time.
• Using Plan Apochromat TIRF series
  -These objectives have the highest N.A. of all Nikon objectives.  Corrected for spherical aberration resulting from temperature-induced changes in the refractive index of the immersion oil from 23°C (room temperature) to 37°C (physiological temperature).
• Plan Apochromat VC objectives

-Excellent resolution up to the edges of the view field and an extremely high image flatness level. Shading has been eliminated by improving the light intensity around the edges of the view field. Axial chromatic aberration has been corrected up to 405nm (h line)—perfect for confocal applications.

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Confocal Laser Scanning Microscope system with spectral image (C1si)

• Effective for observing live cell dynamics. (Simultaneous 32-channel spectral-image
  acquisition)
•  Allows spectral imaging that is gentle on live cells because a wide wavelength spectrum can be captured with a single laser excitation. With accurate fluorescence spectrum and true fluorescence colors can be obtained.
• Wavelength resolution independent of confocal pinhole diameters,* a leak-free laser beam mechanism, sensitivity calibration between fluorescence detectors
• Using a NIST-compliant light source, calibration of optical wavelength transmittance characteristics, and wavelength calibration combine to achieve true spectral imaging.
• Newly developed Diffraction Efficiency Enhancement System (DEES) technology* and high efficiency fluorescence transmission technology* are used in the spectral system.
• Newly developed Dual Integration Signal Processing (DISP) technology* is used in the video signal-processing circuitry to achieve brighter spectral imaging.
• Effective in the observation of expression-multiplexed specimens stained with CFP, GFP, YFP, DsRed and similar reagents, as well as observation of localized protein molecules and FRET observation.
• Separation of autofluorescence is also possible.

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