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Advanced Biological Microscopes



 

• Integration of a laser TIRF system with epi-fluorescence system
  Researchers can observe the same field of view using both the TIRF and epi-fluorescence methods by simply shuttering light sources. Alignment is also extremely easy.
• Ultra-high signal/noise ratio enables observations of single molecules
• High-performance fluorescence cassette holder eliminates the possibility of registration shift in the optical axis for each laser wavelength.
• Simultaneous mounting of other modules such as laser tweezers is possible thanks to the expandable stratum structure of the microscope TE2000.
• Nikon’s laser TIRF system utilizes this evanescent wave to excite single molecules in the thin section in contact with the coverglass. Because the specimen is not excited beyond the evanescent wave, this imaging system can produce fluorescence images with an extremely high signal-to-noise (S/N) ratio.

Available with white-light illuminator supports various types of fluorescence observations The white-light TIRF system enables TIRF microscopy using mercury lamps. By exciting a confined depth, TIRF enables imaging of fluorescence images with a much higher S/N ratio than is possible using the epifluorescence method.

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• Multimode, multiangle viewing of the same field
  The TIRF-C1 system allows multimode imaging with laser TIRF, confocal and epi-fluorescence. This configuration can be a powerful tool for research, allowing the investigation of an event at the cell membrane, and the ability to follow the subsequent event cascade deep into the cells interior

• World’s first oil-immersion-type objectives to incorporate a correction ring for temperature change

  s and coverglass thickness.

• By rotating its correction collar, researchers can easily eliminate spherical aberrations’ negative influence on the image quality resulting from temperature-induced changes in the refractive index of the immersion oil and influence from variation in the coverglass thickness. T
• he lenses have been calibrated for a range from 23°C (room temperature) to 37°C (physiological temperature).
•  Additionally, these objectives also can provide high quality DIC, epi-fluorescence, confocal, and deconvolution imaging, while providing a strong trapping power during applications using laser tweezers.

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