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Andor Widefield Confocal Revolution DSD2 System

The Andor Revolution DSD2 is a simple confocal device delivering extraordinary imaging performance. Laser-free and ease of retrofit to an existing fluorescence microscope, DSD2 offers an affordable confocal option for your own laboratory or core facility. The ease of use is such that you will be imaging in no time!

  • Simple add-on confocal
  • Stunning image quality
  • Large field of view
  • Low running costs (laser-free)
  • Handles broad sample range

 

Revolution® DSD2

Flexibility

The simple design of the DSD2 means that it can be mounted to inverted and upright microscopes and fluorescence stereomicroscopes and macroscopes. With three confocal sectioning options, you can choose the optimal setting for your sample or magnification. This makes the DSD2 probably the most versatile confocal device on the market, addressing the broadest range of sample types. With no fixed laser wavelengths to limit you, you have freedom to choose the best wavelength choice for your research. Fixed or live, there is no limit to the possibilities.

Productivity

With a spinning disk at its core, the DSD2 naturally captures images at higher frame rates than a point scanning confocal. A three dimensional image, at a like-for-like resolution and comparable quality, can be captured in seconds on the DSD2 compared to minutes on a point scanning confocal. This offers two key benefits. Higher throughput of samples, so increasing productivity and earlier data review; and for live samples the ability to capture dynamics in higher temporal detail for greater insight.

Scaleability

The Revolution DSD2 can be used with additional Andor devices from our Photostimulation range. For example, add a MicroPoint to combine confocal imaging with ablation, such as used in damage & repair research, or add a Mosaic 3 for fast targeted illumination to use optogenetic models with DSD2. This simple device can in fact be used for sophisticated applications, now or as your research develops.

Quality

Combining structured illumination and spinning disk technologies with high sensitivity high dynamic range Andor sCMOS cameras, the Revolution DSD2 produces image quality that typically exceeds confocal images captured with laser point scanning systems. This latest generation builds on a field proven device, introducing even better performance and so even higher quality imaging.

Reliability

With no lasers required for the Revolution DSD2, and the simplicity of its design making it an easy device to align, there is no risk of owning a confocal device requiring regular attention to keep it operational. The benefit is worry free imaging for many years, and extremely low maintenance costs.

Features Benefits
Unique Disk Design Delivers images of high contrast with high dynamic range for outstanding results. Even with thick samples.
Confocal flexibility Three confocal sectioning modes to address all magnifications and provide freedom to select best match for sample
Large field of view Image large samples or larger numbers of cells to increase productivity
Broad spectrum, laser free 370 – 700nm excitation with full flexibility of filter choice
Easy alignment and low maintenance Minimal running costs for product longevity.
Optimized software and algorithms Up to 22 frames per second real-time confocal imaging for high productivity or live cell imaging. Intuitive control for fast operation and minimal training
Real-time control and viewing Switch viewing mode between widefield and confocal with one mouse click to ease locating sample
Confocal and Widefield modes Use DSD2 to image even weakly fluorescent samples which are difficult to see in confocal.
Microscope agnostic, including stereo and macroscopes Image using your preferred microscope according to your sample requirements
By-pass mode Remove the disk for higher speed fluorescence imaging
Key Specifications
Imaging 4 filter cube positions
Excitation range 370 – 700 nm
Emission range 410 – 750 nm
Frame rates (max) 22 fps (16-bit, confocal mode, full frame, 1×1 binning)
Confocality (PSF) 3 sectors provide 1.2 μm, 1.0 μm, 0.8 μm (FWHM)
Operating Principal Structured illumination, spinning disk. 3 grid densities for matching sectioning and throughput to objective and sample
Disk rotation speed 3000 rpm
Minimum Exposure 20 ms (1 full disk rotation)
Widefield By-pass Selectable mode completely removes disk from the imaging path
Switching time between disk sectors <3 s
Filter Cubes Turret 4 positions, user-replaceable cubes
Filter switching time <200 ms