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Inverted Microscope Eclipse Ti-U

NIKON Universal inverted microscope that can be configured for use with motorized components.

Flexible specification inverted microscope configurable to meet your research needs
The Eclipse Ti-U inverted research microscope from Nikon is our universal model that can be configured for use with additional motorised components allowing you to build up a microscope to meet many of today’s demanding microscopy techniques. The Ti-U comes in a choice of two models with a standard three port model or expanded four port model which gives you access to a bottom port when your application demands you capture every single photon. The Eclipse Ti-U uses Nikon’s world-famous CFI60 optical system and a wide choice of objectives for bright field, dark field, fluorescence, phase contrast, DIC and modulation contrast microscopy techniques.

High-speed Motorized Control and AcquisitionThe operational speeds of motorized components such as the nosepiece, fluorescence filters and stage have been greatly enhanced, allowing high-speed screening image capture during multi-dimensional experiments. Faster device movement and image acquisition reduce overall light exposure and subsequent photo-toxicity, leading to more meaningful data. The digital Controller Hub significantly increases motorized accessory speed by reducing communication overhead time between components, boosting total operation speed.

 

Ti-E Motorized Control

 

Multipoint snapshots of HeLa cells transiently expressing Venus-tubulin and mCherry-actin and stained with Hoechst33342 and DiD. (All in pseudo-color) Dr. Kenta Saito, Research Institute for Electronic Science, Hokkaido University and Dr. Takeharu Nagai, The Institute of Scientific and Industrial Research, Osaka University

 

High-quality Phase Contrast Images using High NA Lenses

 

Phase Contrast

 

The revolutionary external phase contrast unit enables the use of high N.A. non-phase contrast objective lenses for phase contrast imaging by the use of an external phase ring.  As this external phase ring is not in the epi-fluorescence light path, users can capture both fluorescence images as well as phase contrast images without compromising on fluorescence tranmission.

 

 

 

Advanced Integration with Peripherals via NIS-Elements Imaging Software

Nikon’s comprehensive imaging software NIS-Elements provides an integrated control of the microscope, cameras, components and peripherals. The intuitive GUI and efficient workflow make the programming of automated imaging sequences up to 6D (X, Y, Z, time, wavelength, multipoint) easy to perform.

6D time-lapse imaging system

 

Two-tiered Configuration Capability

Taking advantage of the Nikon Ti’s stratum structure, modules can be incorporated as two separate layers with multiple modules per layer. Using a dual layer configuration enables optimal filter configuration for each illumination module. This enables optimal filter selection and improves experimental accuracy whilst maintaining the highest acquisition speeds.

Two-tiered configuration

 

Multiport Design with a Maximum of Five Imaging Ports
The Ti-U has two built-in imaging ports* that can be dedicated to specific tasks, offering a flexible configuration at a high cost-to-performance ratio.
(* With Ti-U/B model with bottom port)

Main body Port Ti-U: 3 portsEyepiece 100%, left 100%, right 100%, AUX**Ti-U/B: 4 portsEyepiece 100%, left 100%, right 100%**, bottom 100%

Manual optical path switching

Two ports (tube base unit with side port, back port) can be added optionally
Focusing Via nosepiece up/down movementStroke (manual): up 8mm, down 3mmCoarse stroke: 5.0mm/rotationFine stroke: 0.1mm/rotation

Minimum fine reading: 1µm

Coarse refocusing mechanism
Intermediate magnification 1.5x
Other
Eyepiece tube Eyepiece tube body TI-TD Binocular Tube D, TI-TS Binocular Tube S, TI-TERG Ergonomic Tube
Eyepiece tube base TI-T-B Eyepiece Tube Base Unit, TI-T-BPH Eyepiece Tube Base Unit for PH, TI-T-BS Eyepiece Tube Base Unit with Side Port
Eyepiece lens CFI 10x, 12.5x, 15x
Illumination pillar TI-DS Diascopic Illumination Pillar 30W, TI-DH Diascopic Illumination Pillar 100W
Condenser ELWD condenser, LWD condenser, NAMC condenser, ELWD-S condenser, High NA condenser, Darkfield condenser, CLWD condenser
Nosepiece
TI-ND6-E Motorized Sextuple DIC Nosepiece, TI-N6 Sextuple Nosepiece, TI-ND6 Sextuple DIC Nosepiece
Objectives CFI60 objectives
Stage TI-S-ER Motorized Stage with Encoders, TI-S-E Motorized Stage—Cross travel: X110 × Y75 mm, Size: W400 × D300 mm (except extrusions)TI-SR Rectangular Mechanical Stage, TI-SR/F Rectangular Stage with front positioned knob, TI-SSR Short-handle Rectangular Stage-Cross travel: X70 x Y50mm, Size: W310 x D300mmTI-SP Plain Stage—Size: W260 x D300 mmTI-SAM Attachable Mechanical Stage—Cross travel: X126 × Y84 mm when used with TI-SP Plain Stage
Motorized functions
Epi-fluorescence attachment Sextuple fluorescence filter cube rotating turret, Filter cubes with noise terminator mechanism, Field diaphragm centerable, 33mm ND4/ND8 filters, 25mm heat absorbing filterOption: Motorized sextuple fluorescence filter cube rotating turret, Motorized excitation filter wheel, Motorized barrier filter wheel
Nomarski DIC system Contrast control: Senarmont method (by rotating polarizer)Objective side prism: for individual objectives (installed in nosepiece)Condenser side prism: LWD N1/N2/NR (Dry), HNA N2/NR (Dry/Oil) types
Weight (approx.) Phase contrast set: 38.5kgEpi-fl set: 42.3kg
Power consumption (max.) Full set (with HUB-A-U and peripherals): approx. 40W