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Macro Confocal Imaging System AZ-C2+

High-definition macro to micro confocal imaging

Nikon’s large field of view macro confocal imaging system.

With high-definition wide field of view images larger than 1cm with unprecedentedly high S/N ratios, the AZ-C2+ allows for imaging of whole-mount specimens, such as embryos, in a single shot. It offers a combination of low and high magnification objective lenses, optical zoom and a confocal scanning zoom function, enabling continuous imaging from macro to micro. Moreover, the AZ-C2+ allows deep imaging of in vivowhole specimens.

High-definition macro to micro confocal imaging

The AZ-C2+ enables high-definition confocal imaging during macro observation as well as micro observation.

Sharp wide field of view images with unprecedentedly high S/N ratios allow for imaging of whole-mount specimens such as embryos and large tissue slices that are commonly used in developmental biology andsystems biology studies.

Moreover, the AZ-C2+ offers a combination of low and high magnification objective lenses and a scanning zoom function, enabling continuous imaging from macro to micro with a single microscope.

The macro in vivo imaging capabilities allow for the capture of confocal images that were previously not possible with traditional stereoscopic microscopes.

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TT2 ES cells

Anti-Nanog antibody (Cy3), anti-Oct3/4 antibody (Alexa488) and DAPI localized in cell nuclei

Photographed with the cooperation of: Hiroshi Kiyonari, Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology

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Photo courtesy of: Director and Professor Masatoshi Yamamoto, Drosophila Genetic Resource Center, Kyoto Institute of Technology

Comparison of same specimen regions captured by the AZ-C2+ and an epi-fluorescence microscope

The AZ-C2+ eliminates out-of-focus light and flare to deliver highly resolved confocal fluorescence images and optical sections.

C2+ HiDef3
C2+ HiDef4
C2+ HiDef5
C2+ HiDef6

Confocal fluorescence
maximum projection image

Standard wide-field
fluorescence image

Confocal fluorescence
maximum projection image

Standard wide-field
fluorescence image

Specimen: 7.5-day-old mouse embryo expressing H2B-EGFP.
(Plan Fluor 2x used)
Photos courtesy of: Dr. Toshihiko Fujimori*1*2, Dr. Go Shioi*2
*1 Division of Embryology, Developmental Biology, National Institute for Basic Biology
*2 Genetic Engineering Unit, Laboratory for Animal Resources and Genetic Engineering, Center for Developmental Biology, RIKEN
Specimen: Zebrafish eye double-stained with GFP and mCherry
Photos courtesy of: 2008 Physiology Course, Marine Biological Laboratory

One-shot whole specimen macro confocal imaging

High NA objectives for macro observation enable fast, high-resolution, single-image capture of a wide specimen area. Because the objectives cover a field of view larger than 1 cm, imaging of embryos during late stages of development and the dynamics of cell populations in whole organs are possible.

[Note] When Plan Apochromat 1x and AZ100 optical zoom 4x are used, the diagonal diameter of the real field of view is 5.3 mm.

C2+ HiDef7
C2+ HiDef8
Confocal fluorescence maximum projection image (Plan Fluor 2x used) Conventional confocal microscope (stitched image)

The AZ-C2+ can capture wide-field, optical sections at high resolution in a single scan. With a conventional confocal microscope, image stitching is necessary because the field of view that can be captured in a single scan is small.

Specimen: Rat tongue slice
C2+ HiDef9
C2+ HiDef10
Confocal fluorescence maximum projection image (Plan Apochromat 1x used)
Specimen: Neurons (green) and blood vessels (red) of 6.0-day-old chick embryo
Confocal fluorescence maximum projection image (Plan Apochromat 1x used)
Specimen: Blood vessels (red) of 2.5-day-old chick embryo

Photos courtesy of: Dr. Yoshiko Takahashi, Molecular and Developmental Biology, Graduate School of Biological Science, NAIST

AZC2+ HiDef11
AZC2+ HiDef12
Confocal fluorescence maximum projection image (macro image, Plan Apochromat 1x used) Confocal fluorescence maximum projection image (magnified image)

Specimen: Rabbit hyaline cartilage cells embedded in atelocollagen gel and cultured for 21 days; live cells (green) and type II collagen (red)

Photos courtesy of: Dr. Masahiro Kino-oka, Laboratory of Bioprocess Systems Engineering, Department of Biotechnology, Division of Advanced Science and Biotechnology, Graduate School of Engineering, Osaka University

 

AZC2+ HiDef13

The AZ-C2+ allows time-lapse observation of the dynamic behavior of cell populations.

Specimen: Chick embryo in stage IV (Plan Fluor 2x used) expressing GAP43-eGFP to label plasma membrane.
Photos courtesy of: Dr. Yukiko Nakaya, Laboratory for Early Embryogenesis, Center for Developmental Biology, RIKEN

Continuous imaging from low magnification to high magnification
With five different objective lenses, optical zoom and confocal scan zoom, the AZ-C2+ makes imaging possible from very low magnification to high magnification. Macro imaging, such as whole-section imaging, and micro imaging, including imaging of a single cell, can be done using a single microscope.

High magnification imaging offers clear and sharp images of single cells.

Specimen: Human breast cancer cell line MDA-MB-231 (Plan Fluor 5x used)
Photos courtesy of: Dr. Kazuyuki Itoh, Department of Biology, Osaka Medical Center for Cancer and Cardiovascular Diseases
AZC2+ HiDef14

AZC2+ HiDef15

Zoom 1x

AZC2+ HiDef16

Zoom 2x

AZC2+ HiDef17

Zoom 4x

AZC2+ HiDef18

Zoom 8x

Specimen: BPAE cells (Plan Fluor 5x and C1 confocal scan zoom used)

Deep imaging of whole specimens

The AZ-C2+ allows imaging deep into the specimen–difficult to achieve with conventional confocal microscopes. The AZ-C2+ efficiently captures fluorescence signals from deep within a specimen in macro and in vivo imaging.

Nerve cells (red) 2 mm beneath the surface of the embryo can be imaged clearly.

 

DeepImaging

Specimen: 2.5-day-old chick embryo
Photo courtesy of: Dr. Yoshiko Takahashi, Molecular and Developmental Biology, Graduate School of Biological Science, NAIST

 

Confocal laser microscope system C2+/C2si+ Ready/C2si

The Nikon C2+ series offers the optimum confocal system to meet both your research and your budgetary needs.

C2+: Standard model boasts high resolution, high sensitivity, and high contrast. Suited for single laboratories or large research groups.

C2si+ Ready: Upgrade to C2si+ is possible by adding a spectral detector.

C2si+: Spectral confocal system featuring a 32-ch multianode spectral detector. A spectral bandwidth of 320 nm can be captured in a single scan.

AZ-C2+ System

Configuration with spectral confocal system C2si+

[Note] Upgrade to the macro confocal microscope system is through a combination of the C2+ series and the AZ100 microscope. The AZ100M andA1+ series are not compatible.

Sample images captured with AZ-C1

 

AZ100 optical zoom 1-8x (zoom ratio 8 : 1)
C2+ confocal scan zoom 1x-1000x (continuous variable)
Stand AZ-STE EPI stand/AZ-STD DIA stand Focus range: 85 mm stroke

Coarse focus: 18.5 mm/rotation

Fine focus: 3.27 mm/rotation

Stage focus: 10 mm range with 0.27 mm/rotation
Under software control with remote focus accessory

Objective AZ-Plan Apochromat 0.5x (NA 0.05/W.D. 54 mm), AZ-Plan Apochromat 1x (NA 0.1/W.D. 35 mm), AZ-Plan Fluor 2x (NA 0.2/W.D. 45 mm), AZ-Plan Apochromat 4x (NA 0.4/W.D. 20 mm), AZ-Plan Fluor 5x (NA 0.5/W.D. 15 mm)
Laser light source Laser wavelength options 440 nm,488 nm
Ar laser (457nm,477nm488nm,514nm),
543 nm/561nm/594nm
633nm/638nm/640nm
Maximum number 4
Laser control options AOM/AOTF/manual
Laser shutter Motorized mechanical shutter (each laser)
Standard fluorescence detector Number of channels C2si+: 3 channels, C2si+ Ready: 3 channels, C2+: 2 channels/3 channels
Display mode 160 x 160 to 2048 x 2048 pixels
Scanning speed Standard mode:2 fps (512 x 512 pixels, bi-direction)
Zoom:1-1000x
Fast mode:8 fps (512 x 512 pixels, bi-direction)
Zoom:8-1000x
Spectral detector (C2si+) Number of channels 32 channels
1st dichroic mirror 20/80 beam splitter
Corresponding wavelength 400-750 nm
Wavelength resolution 2.5/5/10 nm (switchable)
Minimum wavelength step 0.25 nm
Display mode 160 x 160 to 1024 x 1024 pixels
Scanning speed Standard: 0.5 fps (512 x 512 pixels, 32 channel simultaneous recording )
Power Confocal system (PC, monitor, C2+ controller, AOM controller): Approx. 830 W (single phase AC 115 V, 7.2 A / AC 230 V, 3.6 A, with earth)

(Does not include microscopes and lasers.)