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Microscope Camera Head DS-Qi2 Monochrome Camera

New 16.25-megapixel, high-definition monochrome microscope camera equipped with Nikon FX-format CMOS sensor. Delivers high-sensitivity, low-noise images.

A new 16.25 megapixel monochrome camera that perfectly captures low light fluorescence and large fields of view.

For the first time ever, Nikon has equipped with a Nikon digital SLR camera FX-format CMOS sensor and optimized it for microscopy. The result is an ultra-high quality 16.25 megapixel monochrome camera that features high pixel density, high sensitivity and low noise, making it an excellent choice for applications in quantitative fluorescence imaging.

CMOS

Nikon CMOS sensors and imaging technologies for professional D-SLR cameras have been optimized for microscopy.

High Sensitivity

Detects even faint fluorescent signals

7.3μm pixels, high quantum efficiency, and very low read noise allow the DS-Qi2 to read in even faint fluorescent signals.

quantum-efficiency

Excellent Linearity

Reliable quantitative analysis made possible

With a linearity error of ±1%, the DS-Qi2 is a superb tool for measuring intensities in fluorescence samples, including timebases intensity measurement and radiometric meassurement.

High Frame Rate

Fast focusing, even with fluorescent images

With a high-sensitivity CMOS sensor and USB 3.0-based data transfer, the DS-Qi2 enables high-speed live imaging and image capture at up to 45 fps (1636×1088 pixels).

Low Noise

Acquires dim fluorescent signals with ultra-low noise

2.2 electrons read noise coupled with a large full-well capacity allows the acquisition of fluorescence images with very little noise and very high dynamic range.

low-noise1

low-noise2
LLC-PK1 cells expressing GFP-EB3 tubulin with low noise. Large linear full well capacity allows acquiring both the brightest and dimmest areas in a single capture.
Sample courtesy of: Michael Davidson, National High Magnetic Field Laboratory, Florida State University

 

Time-lapse Photography

Fluorescent time-lapse imaging through integration with NIS-Elements software

With a large field of view and pixel density, and low noise, the DS-Qi2 is ideal for time-resolved imaging applications.

timelapse1

timelapse

Time-lapse images (every 1 second) of LLC-PK1 cells with GFP-EB3 tubulin. Each image represents the maximum intensity projection of the time-lapse, allowing visualization of the end-binding protein located on the microtubule plus-ends, and allowing tracing of the microtubule path.

DS-Qi2 captures an extremely large field of view, but still represents very fine details as demonstrated in this cropped time-lapse sequence from a large FOV image.

Objective: CFI Plan Apochromat λ 60x oil / NA: 1.4

Sample courtesy of: Michael Davidson, National High Magnetic Field Laboratory, Florida State University

With a large field of view and pixel density, and low noise, the DS-Qi2 is ideal for time-resolved imaging applications.

Image sensor Nikon FX-format, Monochrome CMOS senser / Size: 36.0×23.9mmEffective 16.25 megapixels
Recordable pixels 4908×3264 pixel (full-pixel), 1636×1088 pixel (3×3 pixel averaging)
Cooling method Electronic cooling
ISO sensitivity(recommended exposure index) Standard: equivalent to ISO 800 (Selectable from ISO800 to 51200 equivalent
Live display mode Full-pixel 4908×3264 pixel (max 6fps) / 3×3 pixel averaging 1636×1088 (max 45fps)
Lens mount F mount
Exposure time 100 μsec to 60 sec
Image format BMP, TIFF, JPEG, etc., selectable in NIS-Elements
Interface USB3.0 (computer control connection) ×1, External sync input/output ×1
Power supply AC100-240V 50/60Hz
Power consumption 24W
Dimensions 105 (W) × 134 (D) × 153 (H) mm
Weight 1200g
Operating environment 0-30°C, 80% RH max, 30-40°C, 60% RH max. (without condensation)