PicoQuant Upright Time-resovled Fluorescence Microscope – MT100

Upright Time-resolved Fluorescence Microscope

PicoQuant Upright Time-resovled Fluorescence Microscope – MicroTime 100

The MicroTime 100 is an idea tool for the study of time-resolved photoluminescence of solid samples such as wafers, semiconductors or solar cells. The system is based on a conventional upright microscope body that permits easy access to a wide range of sample shapes and sizes. The MicroTime 100 can be supplied with either manual scanning or with a 2D piezo scanner with either µm or cm resolution.

-Complete system with laser combining unit, upright microscope body and detection unitomplete confocal STED system based on a inverted microscope body
-Pulsed diode lasers with wavelengths from 375 nm to 810 nm
-Multiple detector options for up to four detection channels
-XY-scanning piezo stage for 2D-lifetime imaging
-Wide range scanner with a scan range up to several cm

Flexible excitation subsystem

The excitation subsystem of the MicroTime 100 consists of a pulsed diode laser driver of thePDL Series and different laser heads with pulses in the picosecond time regime (additional CW mode is available as an option). The available wavelengths range from 375 to 900 nm. Laser power and repetition rate can be flexibly adjusted by the laser drivers of the PDL Series.
The laser heads are either directly coupled to the MicroTime 100 or integrated along with multiple optical components in one Laser Combining Unit (LCU) for easier handling, attenuation and coupling into an optical fiber.

Laser Combining Unit (LCU) of the MicroTime 200

Detection subsystem with single photon sensitivity

The MicroTime 100 was specially designed for maximum flexibility yet at a very high light collection efficiency. The MicroTime 100 can be configured up to four detection channels which are coupled to the microscope via a multimode fibre. The system can be equipped with a variety of sensitive detectors, which are optimized for wavelength, signal brightness, or photophysical attributes like afterpulsing free detection. The choice of detectors includePMA Series or PMA Hybrid Series detectors and optimized SPADs for efficiency or timing.

Single Detector Unit (SPAD) for MicroTime 100

Timing with picosecond resolution

The data acquisition scheme of the MicroTime 200 is based on the method of Time-Correlated Single Photon Counting (TCSPC) in the unique Time-Tagged Time-Resolved mode (TTTR). Using TTTR data acquisition vastly different measurement procedures ranging from standard lifetime measurements to Fluorescence Lifetime Imaging (FLIM) orTRPL Imaging and even coincidence correlation (“antibunching”) can be performed, based on just one fundamental data format. The TTTR format ist supported by all available TCSPC electronics from PicoQuant. Using these high-end integrated devices fluorescence lifetimes down to a few picoseconds or even up to ms for phosphorescence and luminescence studies can be easily resolved.

PicoHarp 300 - Stand-alone TCSPC Module with USB 2.0 Interface

Intuitive data handling and analysis

Based on the sophisticated data collection and handling, the system softwareSymPhoTime 64 supports a multitude of methods, such as intensity time trace, burst analysis, lifetime histogramming, Fluorescence Correlation Spectroscopy (FCS),Fluorescence Lifetime Correlation Spectroscopy (FLCS), Fluorescence Lifetime Imaging (FLIM), TRPL Imaging and Förster Resonance Energy Transfer (FRET), to name only a few.
SymPhoTime 64 data handling maintains a transparent data structure where all derived data is maintained in one workspace, including a log file to keep track of all measurement and analysis steps.
A large number of algorithms for those methods are already integrated in SymPhoTime 64, providing a analysis platform for ready-to-publish data. At the same time, SymPhoTime 64 offers enhanced flexibility for the integration of novel, cutting edge algorithms by the user. A dedicated scripting language interface allows to modify and expand the analysis routines. In addition to data analysis within SymPhoTime 64, data can be exported to standard formats for external analysis.
Our interactive user forum as well as regularly held SymPhoTime training days offer outstanding support for new and advanced users.

SymPhoTime64 - Time trace analysis

Excitation system	Picosecond diode lasers with adjustable output power and repetition rates up to 80 MHz inside a compact fibre coupling unit Wavelengths between 375 and 900 nm Single or multichannel laser driver New: 560 nm picosecond pulsed excitation with the LDH-D-TA-560
Microscope	Upright microscope BX43 from Olympus Side ports and back port still accessible (for e.g. widefield imaging or TIRF) Transmission illumination unit included Manual positioning stage
Objectives	Air objectives with 20x and 40x magnification (standard) Various high end objectives available (Oil / Water immersion, air spaced, IR/UV-enhanced, TIRF or long working distance objectives upon request
Scanning	Optional 2-dimensional piezo scanning table with 80 µm x 80 μm scan range at nominal 1 nm positioning accuracy, mounted in objective scanning fashion Optional: large area scanning table with centimeter range
Main optical unit	Confocal detection setup in a compact housing with up to four parallel detection channels for PMA and PMA Hybrid detectors, two detection channels for SPAD detectors Prealigned exchangeable major dichroic mirror holder All optical elements easily accessible and adjustable
Detectors	Single Photon Avalanche Diodes Hybrid-Photomultiplier Tubes Photomultiplier Tubes
Data acquisition	Based on the method of Time-Correlated Single Photon Counting (TCSPC) in the unique Time-Tagged Time Resolved (TTTR) measurement mode Simultaneous data acquisition of up to two channels
Software	SymPhoTime 64 for imaging and FCS applications FluoFit software for lifeime measurement applications without scanning

The MicroTime 100 is an upright time-resolved confocal microscope, that can be used to perform several applications such as:

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