IVIM Technology IVM-C3 (Confocal v.3)

The New All-in-One Intravital Imaging Platform

Tractable, Fast and Gentle

IVM-C3 is a highly integrated intravital microscopy for in vivo imaging with an enormously increased detection efficiency, optical resolution, and contrast of the image compared to conventional fluorescence microscopy. Equipped with a 4-wavelength laser and four high-sensitivity confocal detectors, IVM-C3 allows multi-dimensional views of living cells and tissues in 3D or 4D up to four different colors. It is the optimal system for simultaneously observing various dynamic multi-cellular behaviors in small live animal models.

Highlights
Specification

Highlights

Multi-color Simultaneous Imaging (4 channels, 4 different colors)
Fully Integrated in vivo Maintenance Unit / Animal Stage
(e.g., Monitoring & Homeostatic Regulation of Animal Vitality)
Ultra High-Speed Imaging (max. 100 fps – 512×512 pixels)
4D Animal Motion Compensation (XYZ & Time)

Specification

	IVM-C3	IVM-M3	IVM-CM3	IVM-MS3	IVM-CMS3
Laser
Type	Confocal Laser Unit	Tunable Two-Photon Laser Unit Ti:Sapphire laser	Confocal Laser Unit Tunable Two-Photon Laser Unit Ti:Sapphire laser	Compact Two-Photon Laser Unit	Confocal Laser Unit Compact Two-Photon Laser Unit
Wavelength	405 nm (20mW), 488 nm (20mW), 561 nm (20mW), 640 nm (20mW)	690 – 1,050 nm	For Confocal 405 nm (20mW), 488 nm (20mW), 561 nm (20mW), 640 nm (20mW) For Two-Photon 690-1,050 nm	Fixed 920 nm	For Confocal 405 nm (20mW), 488 nm (20mW), 561 nm (20mW), 640 nm (20mW) For Two-Photon Fixed 920 nm
Fluorescence
Detector	Confocal Detector Wavelength: 185 – 900 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.) 4 Ultra-broadband high SNR PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current) 25-2,000 μm variable pinhole	Two-Photon Detector Wavelength: 185 – 760 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.) 4 High quantum efficiency PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)	Confocal Detector Wavelength: 185 – 900 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.) 4 Ultra-broadband high SNR PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current) 25-2,000 μm variable pinhole Two-Photon Detector Wavelength: 185 – 760 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.) 4 High quantum efficiency PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)	Two-Photon Detector Wavelength: 185 – 760 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.) 4 High quantum efficiency PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)	Confocal Detector Wavelength: 185 – 900 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.) 4 Ultra-broadband high SNR PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current) 25-2,000 μm variable pinhole Two-Photon Detector Wavelength: 185 – 760 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.) 4 High quantum efficiency PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)

Variable Emission Filter (Optional): 6 or 2 emission filters can be mounted on each of four detectors

ScanHead

Scanner: Polygonal mirror (Fast axis scanning, Max. 66 kHz)
Galvano scanner (Slow axis scanning, Max. 200 ㎲ /step)

Imaging Head

Objectives: Max. 5 objectives are mountable on S/W controlled motorized turret (1X – 100X)
Compatible for commercial objectives

Image

FOV: 100 x 100 ㎛ ² – 10 x 10 mm²

Pixel Resolution: Max. 2,048 x 2,048 pixels

Imaging Speed: Standard : 30 fps @ 512 x 512 pixels
(Optional) High Speed : 60 fps @ 512 x 512 pixels
(Optional) Ultra High Speed : 100 fps @ 512 x 512 pixels

In Vivo Animal Stage

3D Stage: Travel Range : 50,000 x 50,000 x 75,000 ㎛ (XYZ)
Micromanipulation (Max. 0.2 ㎛ resolution)
3-axis independent control with Jog Dial & S/W

Animal Motion Compensation

4D In Vivo Imaging Motion Compensation: XY motion compensation : Averaged image acquisition with motion artifact compensation
Z motion compensation : Image-based sample Z position adjustment for long-term intravital microscopic imaging & sample tracking (Feedback-loop automatic stage control)
T motion compensation : Image-based image XY position adjustment for long-term intravital microscopic imaging & sample tracking (Feedback-loop automatic stage control)
Combination of above three compensation for 4D in vivo motion compensation
Controllable by IVM Engine software

Additional In Vivo Modules

Live Animal Maintenance Unit: Body Temp. Monitoring & Feedback Heater Control including tablet PC
4CH Rectal Probe, Body Plate Heater, Thermometer Sensor & Cover Glass Heater

In Vivo Imaging Chamber SET: Standard Dorsal Skinfold Chamber SET
Lung Imaging Chamber SET
Cranial Window SET
Abdominal Imaging Window SET
Pancreas Imaging Window SET
Mammary Imaging Window SET
Heart Imaging Window SET

Inhalation Anesthesia System: Rodent Animal Inhalation Anesthesia System
Anesthesia Mask and Connections

Engine & Studio Software

Image Display: Independent 4 single channel display (RGBA channel)
Overlay channel display (Selection among RGBA channel)

In Vivo Imaging Mode: Mosaic imaging (XY), Z-stack imaging (Z), Time-lapse imaging (T)
Time-lapse imaging at Multi-position (T- M),
Time-lapse & Z-stack imaging (TZ),
Time-lapse & Z-stack imaging at Multi-position (TZ- M)

IVIM Technology IVM-C3 (Confocal v.3)

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