Nikon A1 HD25 / A1R HD25

New 25mm FOV

When combined with the Ti2-E inverted microscope, the imaging area of the A1 HD25/A1R HD25 is nearly twice the conventional FOV of 18 mm, enabling the user to obtain significantly more data by capturing more of the specimen in each shot.

The large 25 mm FOV of the A1 HD25/A1R HD25 reduces both the required number of images for stitching large images and image acquisition times, enabling efficient, high-throughput imaging of even large-scale samples. The number of required images can be greatly reduced, in particular for large 3D (XYZ) image stitching.

FOV 25 of A1 HD25/A1R HD25: a total of 24 frames

Conventional FOV 18: a total of 48 frames

The combination of a high-speed resonant scanner and large field of view forms an ideal platform for high-resolution screening assays. It dramatically reduces the time needed to analyze multiple samples and conditions.

Left: A well in the 96-well plate is selected Right: The large FOV enables acquisition of entire wells (with a 4X objective)

Large FOV enables measurement of larger areas and high-throughput analysis.

High definition imaging up to 1K x 1K

1024 x 1024 pixels enables acquisition of high-resolution, high-quality images at lower magnifications, enabling compatibility with a wide range of samples.

Comparison of a large FOV image and 6X zoomed image (1024 x 1024 pixels) of fine structures in a 2 mm brain slice of H-line mouse cleared with RapiClear1.52, SunJinLab.
Image courtesy of: Drs. Ryosuke Kawakami, Kohei Otomo, and Tomomi Nemoto, Research Institute for Electronic Science, Hokkaido University

Low phototoxicity for live cells

High speed imaging capability up to 720 fps, in combination with a large field of view, dramatically increases imaging throughput. This scanning method reduces the exposure time of the sample to excitation light, minimizing phototoxicity and photobleaching.

Galvano scanner

Resonant scanner

Comparison of photobleaching of fluorescent proteins when images are acquired using both galvano and resonant scanners. 3D time-lapse images of trunk vasculature in zebrafish larva expressing LIFEACT-mCherry (probe for F-actin) in endothelial cells were acquired every 30 minutes over a period of 15 hours using a galvano scanner (average of 2 images) and a resonant scanner (average of 64 images).
1024 x 512 pixels, 2X zoom, 100 Z-stack images
Note that photobleaching of LIFEACT-mCherry was dramatically suppressed using the resonant scanner.
Image courtesy of: Shinya Yuge Ph.D., and Shigetomo Fukuhara, Ph.D., Department of Molecular Pathophysiology, Institute of Advanced Medical Sciences, Nippon Medical School

Capture large-scale overview images as well as high magnification images with the same instrument. The 25mm FOV of the A1 HD25/A1R HD25 is effective for observation of large samples, while its 1Kx1K high-definition is ideal for the observation of minute structures.

Stitched overview image of marmoset brain captured with CFI Plan Apochromat Lambda 10X objective and detailed image of dendritic spines captured with CFI SR HP Plan Apochromat Lambda S 100XC Sil objective

A1-DUG-2 GaAsP Multi Detector Unit

The A1-DUG-2 is a 4-channel detector unit equipped with high-sensitivity GaAsP PMTs, allowing acquisition of bright signals with minimal background noise, even when fluorescence is weak or the detector unit is used with a high-speed resonant scanner.

A1-DUVB-2 GaAsP Detector Unit

A1-DUVB-2 is equipped with a high-sensitivity GaAsP PMT and allows spectral imaging using both galvano and resonant scanners. This is a fully tunable emission detector unit capable of spectral imaging with user-defined emission bandwidths as low as 10 nm. Variable bandpass mode and continuous bandpass mode are selectable based on the applications and the images can be spectrally unmixed. The option to add a second fixed bandwidth emission channel enables simultaneous multi-channel imaging.

VB (Variable Bandpass) mode

VB (Variable Bandpass) mode allows maximum 5ch color image

Unmixed Image CB (Continuous Bandpass) mode

CB (Continuous Bandpass) mode allows maximum 32 chspectrum imaging

HeLa cells labeled with five-color fluorescence, Nucleus: DAPI, Vimentin: Alexa Fluor® 488, Lamin: Alexa Fluor® 568, Tubulin: Alexa Fluor® 594, Actin: Alexa Fluor® 633
Specimen courtesy of: Dr. Tadashi Karashima, Department of Dermatology, Kurume University School of Medicine

A1-DUS Spectral Detector Unit

The A1-DUS can acquire spectral images at a high wavelength resolution of at least 2.5 nm. 32 channels of fluorescence spectra (up to a 320 nm wavelength range) can be acquired with a single scan, enabling fast imaging at up to 24 fps (512 x 32 pixels). Simultaneous excitation with up to four lasers enables spectral imaging across wider bands.

It precisely unmixes closely overlapping spectra of fluorescent labels and eliminates autofluorescence. Real-time unmixing during image acquisition is highly effective for FRET analysis.

Spectral and unmixed images of five-color-fluorescence-labeled HeLa cells
Specimen courtesy of: Dr. Tadashi Karashima, Department of Dermatology, Kurume University School of Medicine

The unique V-filtering function, with its capacity for filter-less intensity adjustment, allows arbitrary spectral ranges to be selected from 32 channels and combined to perform the filtering function of up to four filters, enabling imaging with the optimal intensity of each fluorescence probe.

Nikon provides a broad range of high-NA objectives with unrivaled optical quality to redefine the boundaries of confocal imaging. Options include silicone oil immersion objectives for thick live cell imaging, large-FOV low-magnification objectives and easy-to-use dry objectives. Chromatic aberrations are corrected from ultraviolet to near infrared range, enabling excellent multicolor imaging.