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Phi Optics – SLIM

Phi Optics Spatial Light Interference Microscopy  (SLIM) comes in three different versions: SLIM Basic, SLIM Pro and SLIM Ultimate. SLIM Basic works with manual microscope stands and is suitable for single-location, single-focus time-lapse imaging. SLIM Pro interfaces with motorized microscope stands to yield fully automated high-throughput, multichannel acquisition. It is suitable for more complicated projects that require 2D and 3D scanning, multi-channel experiments and fast dynamics measurement. At full speed, SLIM Pro generates more than 1TB of data per hour. For efficient management of this high volume of data, SLIM Ultimate combines SLIM Pro with a vast storage server of 100TB.

PHI OPTICS TECHNOLOGY PROVIDES:

  • Real-time quantitative phase imaging and display
  • Non-invasive, label-free cell and tissue imaging on broad time scales (seconds to weeks)
  • Programmed 2D and 3D scanning for large area 2D SLIM maps and 3D SLIM images
  • Seamless overlay with other microscopy channels
  • Quick and easy segmentation of cells

Real-Time Imaging Yes
Programmed 2D Scanning No
Programmed 3D Scanning No
Programmed Fluorescence Overlay No
SLIM Acquisition Speed: Up to 4 fps (1928 x 1448)
Camera: Point Grey USB CCD, 1928 x 1448 Resolution, 26 fps,14 bit
Computer & Storage: Intel Core i7, 16GB RAM, 1TB HDD, NVIDIA GeForce GTX 780, Windows 7
Software: Acquisition: CellVista Basic Postprocessing: ImageJ Phi Optics Toolbox
Hardware Footprint: 43 x 36 x 11 cm / 17 x 14 x 4 in (L x W x H)
Measurement Resolution: Path Length Sensitivity < 1 nm
Transverse Resolution – diffraction limited (e.g. 350 nm with 63X/1.4NA objective)
Axial Resolution – optical sectioning (e.g. 890 nm for 63X/1.4NA objective)

Real-Time Imaging Yes
Programmed 2D Scanning Yes
Programmed 3D Scanning Yes
Programmed Fluorescence Overlay Yes
SLIM Acquisition Speed: Up to 12 fps (2048 x 2048)
Camera: Hamamatsu ORCA-Flash 4.0V3, 2048 x 2048 Resolution, 100 fps, 16 bit
Computer & Storage: Intel Core i7, 32GB RAM, 1TB SSD + 2TB HDD, NVIDIA GeForce GTX 780, Windows 7
Software: Acquisition: CellVista Pro Postprocessing: ImageJ Phi Optics Toolbox
Hardware Footprint: 43 x 36 x 11 cm / 17 x 14 x 4 in (L x W x H)
Measurement Resolution: Path Length Sensitivity < 1 nm
Transverse Resolution – diffraction limited (e.g. 350 nm with 63X/1.4NA objective)
Axial Resolution – optical sectioning (e.g. 890 nm for 63X/1.4NA objective)

Real-Time Imaging Yes
Programmed 2D Scanning Yes
Programmed 3D Scanning Yes
Programmed Fluorescence Overlay Yes
SLIM Acquisition Speed: Up to 12 fps (2048 x 2048)
Camera: Hamamatsu ORCA-Flash 4.0V3, 2048 x 2048 Resolution, 100 fps, 16 bit
Computer & Storage: Intel Core i7, 32GB RAM, 2TB SSD + 100TB HDD, NVIDIA GeForce GTX 780, Windows 7
Software: Acquisition: CellVista Pro Postprocessing: ImageJ Phi Optics Toolbox
Hardware Footprint: 43 x 36 x 11 cm / 17 x 14 x 4 in (L x W x H)
Measurement Resolution: Path Length Sensitivity < 1 nm
Transverse Resolution – diffraction limited (e.g. 350 nm with 63X/1.4NA objective)
Axial Resolution – optical sectioning (e.g. 890 nm for 63X/1.4NA objective)

 

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