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Bioptechs – FCS2/ FCS3/ FCS4 System

Bioptechs chambers offer an easy-to-use, sophisticated solution for high resolution imaging. With an emphasis on what matters most to your lab.

The FCS2 Chamber System the most precise micro-environmental control system on the market. The FCS2 is a parallel plate, high N.A. compatible, uniformly temperature controlled microscopic flow cell with a user definable specimen area for inverted microscopes. Intelligent feedback thermal loop prevents overshoot on temperature and protects the integrity of the sample.

 

Specifications

 

Instructions

FCS2 Chamber Instructions

FCS2 Perfusion Config

FCS2&3 Controller

 

 

 

FCS2 Chamber Features

  • Closed system so that bicarbonate CO2 or organic buffers can be employed
  • Compatible with 1/16″ tubing for perfusion (C-Flex, Tygon, etc.)
  • Easily assembled with ordinary skill (no tools required)
  • Stand-alone temperature controller with an alarm circuit to safeguard your cells
  • Near laminar flow

The Focht Chamber System 3 (FCS3 ®) is a closed system, live-cell micro-observation flowcell chamber that offers several advantages over other chambers. In addition to its unique perfusion and thermal control system, it is fully compatible with all modes of microscopy. It is also the only chamber to combine high-volume laminar flow perfusion rates with Koehler illumination and precise temperature control without an air curtain. Its user definable flow characteristics make it adaptable to nearly any protocol where cells are perfused in a flow-cell.

The FCS3 is a flow cell or flow chamber that is designed specifically for the demands of today’s live-cell imaging requirements. It has limitless flow characteristics because its flow geometry can easily be customized by the user. It can provide low shear near laminar flow as well as high shear in a directed flow and anything in between. The most important feature of the FCS3, other than unequalled temperature uniformity, is that there is a precise directed flow of media over the cells. Open dish type chambers do not provide the flow capabilities of the FCS3. The FCS3 provides an optical imaging cavity where the user can precisely define the optimum flow characteristics necessary for the experiment.

 

Specifications

 

 

 

Instructions

FCS3 Chamber

FCS2&3 Controller

 

 

 

 

The FCS4® Flow Chamber is a versatile, easy to use solution for sample isolation with perfusion flow control that is compatible with all forms of microscopy.    The FCS4 Flow Chamber has all the same features as the FCS2 Flow Chamber with a new ergonomic design for a simplified assembly and adjustable compression.

 

 

Specifications

 

 

Features

  • Ion concentration studies (calcium, chloride, magnesium, etc.)
  • Metabolic studies
  • Cytotoxicity & apoptosis assays
  • Biochemical mechanism studies
  • Drug response studies
  • FISH with chemical (not heat dependent) hybridization
  • SeqFISH with chemical (not heat dependent) hybridization
  • MERFISH with chemical (not heat dependent) hybridization
  • Particle tracking
  • Protein trafficking
  • Vesicle trafficking
  • Membrane transport studies
  • Protein interaction

 

 

Definable flow characteristics

  • Complete control of volume within optical cavity
  • Save on expensive reagents
  • Adjustable separation between optical surfaces
  • Laminar or customizable flow
  • User definable shear force

 

 

 

Advanced imaging compatibility

  • Compatible with all modes of microscopy
  • Adapts to all brands of microscopes
  • ideal for high resolution imaging
  • Accommodates adherent cells, tissue, or suspended specimens

 

 

 

Ergonomic design

  • Self aligning assembly
  • No tools twist assembly
  • Easy to read dial for compression
  • Retro-compatible with all FCS2 Stage adapters