PicoQuant Compact Upright Widefield Photoluminescence Microscope-FluoMic

FluoMic

Compact Upright Widefield Photoluminescence Microscope

Widefield time-resolved photoluminescence microscope
Based on time-correlated single photon counting (TCSPC)
Lifetime detection range from a few ps to several ms with ps timing resolution
Selectable excitation wavelengths from 375 to 1060 nm
Detection range from 400 to 1700 nm
Easy data acquisition and powerful analysis with EasyTau 2 and SymPhoTime 64 software

In many cases, characterizing materials such as semiconductors or solar cells requires measuring their photophysical properties at various points with spatial resolution. Depending on the sample, scanning over a large sample area is often interesting, besides measurements at various points. The FluoMic provides a fast, easy, and reliable way to measure steady-state (from UV to NIR) and time-resolved (from ps to ms) luminescence with a spatial resolution down to 2 μm.

Thanks to its pre-aligned optical fibers, pulsed and CW laser light sources can be used to excite a sample under the microscope. In combination with the FlexWave Wavelength Selection Unit and the SymPhoTime 64 software, steady-state photoluminescence spectra as well as wavelength-selected decays and even time-resolved emission spectra (TRES) can be measured from a small area with FluoMic.

It is available with various microscope stands (e.g., the Olympus BX43, BX53(-P), BX63 and even BXFM) and by this provides a high flexibility for samples and measurement conditions.

Microscope stands ^a)
Olympus	BX43	BX53-P	BX63	BXFM
Type	Upright, manual	Upright, manual	Upright, automated	Upright, manual
Illumination	Transmission, Side-on Epi-Fluorescence	Transmission, Polarization Epi-Fluorescence	Transmission, Epi-Fluorescence	Epi-Fluorescence
Camera ^a)	CMOS, 1280×1060 Pixel, vis-NIR	CMOS, 1280×1060 Pixel, vis-NIR	CMOS, 1280×1060 Pixel, vis-NIR	CMOS, 1280×1060 Pixel, vis-NIR
Dimensions (base unit) (w × d × h) ^b)	400 x 600 x 600 mm	400 x 600 x 600 mm	400 x 600 x 600 mm	400 x 600 x 600 mm
Weight (base unit) ^b)	15 kg	20 kg	25 kg	20 kg
Olympus Objectives ^a)
Magnification	10x	20x	40x	100x
Numeric aperture	0,40	0,40	0,65	0,90
Working distance	3.1 mm	1.2 mm	0.6 mm	1.0 mm
Spot size (excitation)	120 µm	60 µm	30 µm	12 µm
Spot size (emission)	20 µm	10 µm	5 µm	2 µm
Excitation sources
Light source	Laser Diode Heads (LDH Series)		ps Laser Module (Prima/Unico)
Wavelength range	375 – 1060 nm		405, 450, 488, 515, 640 nm
Pulse width range	< 40 – 200 ps, 6000 ps for MB lasers		< 80 – 200 ps
Repetition rate	1 Hz – 100 MHz		1 kHz – 200 MHz
Operation modes	Pulsed, CW, and burst mode		Pulsed, CW, and fast switched CW mode
Detectors^a)
PMT based	PMA-C 175	PMA-C 192	NIR-PMT 1400
Spectral range	230 – 700 nm	230 – 920 nm	900 – 1400 nm
Dark counts (at 20°C)	< 50 cps	< 1100 cps	< 10 000 cps
TTS (FWHM)	< 180 ps	< 180 ps	< 370 ps
Recom. max. count rate	< 5.0 MHz	< 5.0 MHz	< 1.5 MHz
PMA Hybrid	PMA Hybrid-07	PMA Hybrid-40	PMA Hybrid-50
Spectral range	220 – 850 nm	300 – 720 nm	< 370 – 920 nm
Dark counts (at 20°C)	< 150 cps	< 150 cps	< 600 cps
TTS (FWHM)	< 50 ps	< 120 ps	< 160 ps
Recom. max. count rate	< 80 MHz ^d)
SPAD based	MPD	Excelitas	InGaAs MPD
Spectral range	< 400 – 1000 nm	< 400 – 1060 nm	< 850 – 1700 nm
Dark counts (at 20°C)	< 100 cps	< 100 cps	< 400 cps
TTS (FWHM)	< 50 ps (typ. < 35 ps)	< 250 ps (typ.)	< 50 ps (typ. < 35 ps)
Recom. max. count rate	< 10 MHz	< 35 MHz	< 10 MHz
TCSPC electronics
TCSPC device	PicoHarp 330	MultiHarp 150 4P	MultiHarp 150 4N	TimeHarp 260 Pico
Number of channels	1 + 4^e)	1 + 4	1 + 4	1 + 2
Min. bin width	1 ps	5 ps	80 ps	25 ps / 2.50 ns (MCS)
Max. number of time bins	65 536	65 536	65 536	32 768
Full scale time range	65 536 ps – 550 ms	327 ns – 2.74 s	5.24 µs – 22.00 s	819 ns – 170 s (MCS)
Interface	USB 3.0	USB 3.0	USB 3.0	PCIe 2.0 x1
Scanning (optional)
Scanner	Widerange scanner	Widerange scanner	Objective scanner
Type	XY Piezo widerange	XY Piezo widerange	XYZ Piezo objective
Range	75 x 75 mm	25 x 25 mm	80 x 80 x 80 µm
Min. step size	100 nm	75 nm	1 nm
Positioning accuracy	600 nm	300 nm	10 nm
Coupling (optional)
Type	FlexWave	FluoTime 250	FluoTime 300
Operation conditions
PC requirements	Quad-core CPU > 3 GHz, RAM >= 4 GB
Operation system	Windows™ 10/11
Power requirements	220/240 or 110/120 VAC, 50/60 Hz
Table	BX43: regular	BX53: regular	BX63: regular	BXFM: opt. Breadboard
Housing	available on request

a) others on request
b) without breadboard and housing
c) values provided by Hamamatsu
d) with CW excitation
e) upgradable

All Information given here is reliable to our best knowledge. However, no responsibility is assumed for possible inaccuracies or omissions. Specifications and external appearances are subject to change without notice.

Add-on: FlexWave Wavelength Selection Unit for Widefield Microscopes

Feature highlights:

Spectral scanning with > 80 % transmission from 400 to 1000 nm for highest sensitivity
High temporal resolution without pulse broadening by an optical diffraction grating

Suitable for various applications:

Materials Sciences: time-resolved and spectrally resolved microscopic characterization of solar cells and photovoltaics, LEDs and OLEDs, nanoparticles and 2D materials
Measure photoluminescence spectrum at a point of interest in sample
TRES imaging: measure TRPL images at defined emission wavelengths
Measure wavelength-dependent antibunching

Detailed specifications are included in the datasheet.

Specifications:

Spectral range: 400 – 1000 nm
Transmission: > 80 % over the whole spectral range
Bandwidth min.: 1 nm
Bandwidth max.: 80 nm
Step width min: 0.1 nm
Detection: single detector directly mounted or fiber-coupled multi-channel detection unit
Detector types: PMA Hybrid series or SPADs
Software: SymPhoTime 64