Remarkably simple. Precisely yours.
PicoQuant has acquired 25 years of experience with time-resolved single photon counting. During this time, PicoQuant has also cultivated strong ties to the scientific community, which we value immensely. We have gathered the extensive knowledge both of our scientific staff and expert scientists, and merged it to create our best time-resolved instrument & software package so far: Luminosa.
QUALITY AND PRECISION YOU CAN TRUST
Optimal performance for single molecule investigations in every measurement context: One-click auto-alignment procedure even without requiring a sample. Galvo scanning (maximum speed) and objective scanning (maximum photon detection efficiency) on the same microscope.
SAVE TIME AND SIMPLY FOCUS ON YOUR SAMPLES
Context-based, intuitive workflows guide you to efficiently harness the full power of smFRET, FCS and FLIM with confidence. Get analysis results with minimal user interaction. GPU-based algorithms provide fast and reliable results.
ADVANCED FLEXIBILITY
Adjust the observation volume to match the dynamics of your FCS and smFRET assays with a single click. An open mode of operation is available for full access to every optomechanical component via software.
- Software-controlled confocal system based on an inverted microscope
- Versatile excitation system with laser wavelengths from 375 to 1064 nm
- VarPSF: fine-tuned observation volume for FCS and single molecule FRET experiments
- Motorized positioning table for “tiling and stitching” in transmission and FLIM mode
- Scanning options: FLIMBee galvo scanner and piezo objective scanning
- Up to 6 truly parallel detection channels with SPAD and/or Hybrid PMTs
- < 700 ps dead time per channel and 5 ps time bins
- One-click auto-alignment for consistent optimal performance
- Fast results with minimal user interaction thanks to GPU accelerated algorithms and context-based workflows for FCS, FLIM, and single molecule detection
Software
The Luminosa software includes single molecule detection, FCS, and time-resolved imaging methods, as well as the possibility to flexibly define custom measurement and analysis modes. Its design permits quick and easy workflows for experiments, which allow the users to focus on their samples. Clearly arranged interfaces showing only parameters relevant for each application promote reproducible measurements yielding consistent datasets.
Simply select the fluorophores in use, and the software automatically configures excitation lasers, the beampath and the detection. It takes care of parameters important for time-resolved measurements, such as laser repetition rate, and pulsed interleaved excitation. Several online previews of the data during acquisition enable the users to instantly check the sample and data quality, saving precious instrument time. Users can explore their data immediately thanks to fast GPU-based analysis routines.
Feature highlights
LumiFinder:
Automated detection and measurement of immobilised emitters
FRETcompass:
Automated determination of correction factors used in single molecule FRET experiments based on the benchmark study published in 2018
InstaFCS:
Online FCS fitting with automatic suggestions for model and parameters.
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- *Provides first suggestions for FLIM species separation without user interaction
- *Suggested parameters can be fine-tuned
- *Simultaneous TCSPC and phasor analysis options for ROI determination
Sample-free auto-alignment:
Ensures optimal performance for every measurement, even the most challenging ones
CalEx:
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- *Excitation laser power calibration allows setting and displaying excitation intensity in μW
- *No external power meter required
- VarPSF:
- Switch between diffraction limited and larger observation volume