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PicoQuant Fluorescence Lifetime Spectrometer – FluoTime 250

PicoQuant Fluorescence Lifetime Spectrometer – FluoTime 250 integrates all essential optics and electronics for time-resolved luminescence spectroscopy in a compact, fully automated device.

Compact and Modular Fluorescence Lifetime Spectrometer

Fully automated compact and modular system

  • Filter based emission selection, optional monochromator
  • Supports both TCSPC and Multi-Channel Scaling data acquisition
  • Step by step application wizards and scripting option
  • Covers lifetime ranges from picoseconds to milliseconds

A compact spectrometer for routine and complex measurements

PicoQuant Fluorescence Lifetime Spectrometer – FluoTime 250 integrates all essential optics and electronics for time-resolved luminescence spectroscopy in a compact, fully automated device. This spectrometer is designed to assist the user in carrying out routine as well as complex measurements easily and with high reliability thanks to fully automated hardware components and a versatile system software featuring wizards providing step-by-step guidance.

Fully automated with high performance optics

FluoTime 250 - Fully automated lifetime spectrometer with high performance optics

All components (attenuators, polarizers and filter wheel) in the optical beam paths are motorized and controlled by the EasyTau 2 software. Emission wavelength selection is implemented via a motorized filter wheel equipped with various cut-off or bandpass filters as selected by the customer. An optional monochromator is available for the UV/VIS spectral range.

Flexible excitation sources

PicoQuant's PDL 820 laser dirver with LDH laser head and pulsed diodes from the PLS Series

The FluoTime 250 uses picosecond pulsed lasers diodes and LEDs from PicoQuant’s LDH and PLS series, covering a spectral range from 255 to 1550 nm. All excitation sources are controlled via a PDL 820 laser driver, which allows not only to vary output power and repetition rates (up to 80 MHz) but also supports burst mode for selected laser heads.

TCSPC and MCS based data acquisition

TimeHarp 260 - TCSPC and MCS board with PCIe interface

Several outstanding data acquisition units are available with various temporal resolutions (from 4 ps to 250 ns). Coupled with the right detectors from the PMA or PMA Hybrid Series, the FluoTime 250 is capable of measuring time-resolved emissions with lifetimes ranging from approximately 10 ps up to several hundreds ms.

A choice of detectors

Hybrid photomultiplier detector assembly from the PMA Hybrid Series

The FluoTime 250 can be equipped with a single photon counting detector from either the PMA or PMA Hybrid Series.These detectors offer picosecond temporal resolutions and cover different spectral ranges between 180 and 920 nm. For studies in the near infrared, a PMT module with sensitivity from 950 to 1400 nm is also available. Each detector includes an electro-mechanical shutter, active cooling, and overload protection.

System software EasyTau 2

Screen shot the EasyTau 2 software analyzing the fluorescence decay of antracence

The EasyTau 2 software package is the one-stop solution for both full hardware control of the FluoTime 250 as well as for interactive data analysis and fitting. The software offers a Windows-based graphical user interface with dedicated applications wizards guiding users through the optimization and data acquisition process for many common applications. Advanced users get full control over all instrumental aspects thanks to a customized mode and scripting language. The integrated analysis and fitting module supports a broad range of time-resolved spectroscopy applications, such as fluorescence and phosphorescence decays, or anisotropy measurements. A powerful report generator allows creating presentation-ready graphical and numerical output.



Optical configuration
  • L-Geometry
Mode of operation
  • Time-Correlated Single Photon Counting (TCSPC)
  • Multichannel Scaling (MCS)
  • Down to 10 pM (as measured with a coumarin sample, with excitation at 400 nm and emission detected at 500 nm)
Lifetime range
  • < 40 ps to 10 µs with PMT detector and TCSPC electronics
  • < 10 ps to 10 µs with hybrid detectors, TCSPC electronics, and suitable laser
  • Up to several 100 ms with any detector and MCS electronics
Excitation sources
  • Picosecond pulsed diode lasers or LEDs ranging from 250 to 1550 nm with repetition rates up to 80 MHz, common driver unit
Optional monochromator
  • Czerny-Turner type, single monochromator
  • Focal length: 150 mm, one exit slits
  • Stray light rejection typically 1:10-5
  • Photomultiplier Tubes (PMTs) with various spectral ranges and sensitivities between 185 nm and 920 nm
  • Hybrid-PMT with various spectral ranges and sensitivities between 200 nm and 900 nm
  • Infrared sensitive Photomultiplier Tube (PMTs)  between 950 and 1400 nm
  • System control, data acquisition and analysis via a single software interface
  • Clearly structured data storage workspace, with export and arithmetic features
  • Assisted mode using Wizards for commonly used measurements
  • Customized mode with full hardware control
  • Scripting mode for automation, including remote execution of scripts (extension of automation to 3rd party devices)
  • Lifetime analysis based on numerical reconvolution procedure, up to 5th exponential decay functions, lifetime distributions, anisotropy, global analysis, rigorous error analysis based on the bootstrap method