PicoQuant Laser Scanning Microscopes LSM Upgrade Kit

Compact FLIM and FCS Upgrade Kit for LSMs

PicoQuant Laser Scanning Microscopes LSM Upgrade Kit
Confocal Laser Scanning Microscopes (LSMs) are widely used tools in biochemistry, cell biology, and other related life sciences.

rapidFLIM – Redefining standards for dynamic FLIM imaging
FLIM, FRET, and FCS in one turn-key system
Compact, easy-to-use and maintenance-free kit, customized to all major LSMs in various configurations for unlimited flexibility
Highest sensitivity with up to 4 detection channels
Fluorescence lifetimes from < 100 ps up to µs
Advanced and user-friendly data analysis software with multiple analysis tools
Options for anisotropy measurements and deep-tissue FLIM imaging

Excitation System	Picosecond diode lasers with adjustable output power and repetition rates up to 80 MHz inside a compact laser combining unit Wavelengths between 375 and 900 nm Single or multi-channel laser driver Optional: external laser (e.g. Titanium:Sapphire laser) New: 560 nm picosecond pulsed excitation with the LDH-D-TA-560
Supported LSMs	Nikon: A1, C2+, C2, C1si, C1 Leica: TCS SP8, TCS SP5 Olympus: FluoView FV3000, FVMPE-RS, FluoView FV1200 (MPE), FluoView FV1000 (MPE) Zeiss: LSM 880, LSM 780, LSM 710
Detection	Up to four parallel detection channels Descanned or non-descanned configuration Connection via optical fibers to the LSM
Detectors	Hybrid-Photomultiplier Tubes Single Photon Avalanche Diodes Photomultiplier Tubes
Data acquisition	Based on the method of Time-Correlated Single Photon Counting (TCSPC) in the unique Time-Tagged Time Resolved (TTTR) measurement mode Simultaneous data acquisition in up to four channels
Software	SymPhoTime 64

Excitation wavelengths: 405 – 900 nm (FV3000) and 375 – 900 nm (FV1200 / FV1000)
Simultaneous usage of pulsed lasers from PicoQuant and CW lasers from Olympus, independent of excitation wavelength
Usage of pulsed lasers with normal or SIM scanner
Attachment of PicoQuant detectors to a special exit port of the LSM
Parallel setup for confocal and NDD imaging (FV1000MPE, FV1200MPE) as well as polarization measurements
Data analysis using the powerful SymPhoTime 64 software
Usage of up to four internal NDD PMTs for deep-tissue FLIM (FV1000MPE, FV1200MPE)

For details, please check the selection chart for FluoView FV3000, FluoView FV1000 (MPE) / FV1200 (MPE), and FVMPE-RS.

Excitation wavelengths: 375 nm – 900 nm
Parallel attachment of pulsed lasers from PicoQuant and MPE laser (Nikon A1)
Simultaneous usage of pulsed lasers from PicoQuant and CW lasers from Nikon A1
Special fiber switch for incoupling of the pulsed lasers (Nikon C series)
Attachment of PicoQuant detectors to a special exit port of the LSM (Nikon A1)
Alternative coupling of PicoQuant detectors and Nikon´s spectral detection unit (Nikon C1si, C2) or Nikon´s PMT detection unit (Nikon C1, C2)
Parallel setup for confocal and NDD imaging as well as polarization measurements (Nikon A1)
SymPhoTime 64 integration into the LSM NIS software to facilitate FLIM and FCS data acquisition
Data analysis using the powerful SymPhoTime 64 software

For details, please check the selection chart for Nikon A1, C1si and C2+, C1 and C2.

Excitation wavelengths: 405 nm – 640 nm
Parallel attachment of pulsed lasers from PicoQuant and Zeiss
Parallel attachment of pulsed VIS lasers from PicoQuant and MPE laser
Attachment of PicoQuant detectors to a special exit port of the LSM
Simultaneous coupling of PicoQuant detectors and the ConfoCor Unit (LSM 710)
NDD in combination with NLO for upright and inverse microscopes
Single as well as dual channel NDD (LSM 710 NLO / LSM 780 NLO)
Parallel setup for confocal and NDD imaging as well as polarization measurements
Data analysis using the powerful SymPhoTime 64 software

For details, please check the selection chart for Zeiss LSM 710 / 780 / 880.

Excitation wavelengths: 405 nm, 440 nm, 470 nm and 640 nm (TCS SP5 / SP8)
Simultaneous usage of pulsed lasers from PicoQuant and CW lasers from Leica
Parallel attachment of the pulsed 405 nm laser from PicoQuant and MPE laser
Spectral FLIM and anisotropy measurements
Attachment of PicoQuant detectors to a special exit port of the LSM
Usage of up to four internal NDD detectors for deep-tissue FLIM
Seamless integration of FLIM and FCS data acquisition using the Leica LASAF software
Data analysis using the powerful SymPhoTime 64 software