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HT-X1™ mini

A compact design with flexible configuration, delivering uncompromised quality and making advanced Holotomography imaging accessible for every lab.
The HT-X1™ mini makes advanced holotomography accessible to all. Compact, intuitive, and ready for any lab bench, it transforms everyday research into breakthrough 3D imaging. Its portable, space-saving design allows effortless installation even in limited environments such as cell culture rooms, clinical labs, or field labs.

Powered by the same 2nd generation holotomography technology as the HT-X1 series, it delivers high-resolution, label-free 3D imaging of live cells. Fully compatible with TomoAnalysis™, the HT-X1™ mini ensures the same high-quality image analysis as the HT-X1 series, providing seamless continuity in research.

The HT-X1™ mini is built to grow with your research. Start with the essentials, then expand capabilities with four upgrade modules — fluorescence imaging, environmental incubation, laser-based autofocus, and multi-wavelength illumination. This modular flexibility lowers initial investment barriers while enabling your system to adapt to new experimental needs — supporting everything from routine cell culture checks to complex, high-content 3D imaging.

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Everyday Access to Advanced Imaging

The HT-X1™ mini integrates advanced holotomography into everyday research workflows with its compact footprint and intuitive operation. Designed for space-limited environments — from cell culture rooms to mobile field laboratories — it can be positioned in routine experimental areas for convenient access to imaging within your workflow. This proximity enables quick checks such as cell confluency monitoring. With holotomography’s label-free capability, researchers can observe cells in their natural state, track cell count changes over time, and continue culturing the same samples — integrating everyday tasks into the imaging workflow while maintaining sample integrity.

 

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Fully Compatible with TomoAnalysis™

The HT-X1™ mini delivers similar high-resolution, label-free 3D imaging quality to the flagship HT-X1™ series — ensuring that every dataset is directly comparable across the HT-X1™ family. All analysis is powered by TomoAnalysis™, Tomocube’s AI-driven analysis platform. Researchers can perform advanced, automated workflows including nuclei detection, cell volume segmentation, and lipid droplet quantification. For mitochondria, the software provides detailed analysis of morphology and network connectivity, capturing structural changes that are critical for studying cellular dynamics. The software also supports various sample types such as organoids, blood cells, yeast, and bacteria, with options to integrate fluorescence imaging data. By combining consistent imaging with a unified analysis ecosystem, the HT-X1™ mini enables reproducibility, seamless collaboration, and confidence that every experiment meets the same high-quality standard.

 

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Compact Design with Expandability

The HT-X1™ mini is an accessible system that can be easily introduced into any individual laboratory, from small research groups to multi decipline institutions. It combines a compact footprint with flexible expandability, allowing researchers to start simple and add advanced modules — such as fluorescence imaging, long-term time-lapse with environmental control, laser-based autofocus, and additional wavelengths — as their research evolves. This makes it a future-proof model that grows with the needs of the lab while maintaining ease of use and space efficiency. With this adaptability, researchers can perform label-free 3D live-cell imaging in virtually any setting, including cell culture rooms and shared facilities. By lowering the barriers to adoption and offering scalability without compromise, the HT-X1™ mini empowers scientists to gain deeper insights, accelerate discoveries, and align advanced imaging capabilities precisely with their experimental goals.

Immunology


Cell biology

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3D biology

 

Cell biology

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Regenerative medicine

Cell biology

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Cell biology

Cell biology

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HT-X1 mini

Instrument

Dimensions 430 (W) x 526 (D) x 492 (H) mm
Weight Approx. 30kg
Power supply 100-240 VAC, 50/60 Hz, 5-3A
Stage travel range 85 x 60 mm
Objective lens 40x NA 0.75 air
Objective working distance 510 μm
Condenser lens NA 0.72
Image sensor 2.8 Megapixels CMOS
Field-of-view 218 μm × 165 μm
Autofocus (optional) Laser-assisted active sensor
Imaging modalities Holotomography, Brightfield(option), Max. 3 Fluorescence(option)
Compatible labware *TomoDish 35 mm

*TomoDish 50 mm

*Microscopic slide(#1.5 bottom thickness)

*Chamber slide(#1.5 bottom thickness)

 

Holotomography Optics

Light source LED
Illumination wavelength 444 nm, 520 nm (option), 660 nm (option)
Axial scan range 31, 62, 146 µm (for bacteria, cells, organoids)
Minimum acquisition speed 1.3 sec per image

 

 

Fluorescence Light Engine (optional)

Light source LEDs
Excitation filters 378/52, 474/27, 554/23 (nm)
Emission filters 432/36, 515/30, 595/31 (nm)
Fluorescence light source trigger 3 channels

 

 

Environmental Controller (optional)

Dimensions 110 (W) x 208 (D) x 206 (H) mm
Weight 3.8 kg
Power supply 100-240 VAC, 50/60 Hz
Temperature control 30 – 40℃
CO₂ range 5 – 20%
Humidity control 5 – 20%

 

 

Ordering information

Cat. No.900031-01 HT-X1™ mini Holotomography System

 

 

TomoDish™ 50 mm

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TomoDish™ 35 mm

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Vessel holder for HT-X1 mini

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