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Holotomographic microscopy HT 2

The HT-2 series opens a new era of 3D correlative imaging, combining the holotomography and fluorescence methods.
The HT-2 allows the viewing of a 3D RI tomographic image of a living cell with minimal damage, while at the same time targeting consumer interest by allowing for fluorescence labeling in the vary same cell.


HT-2 : Holotomographic microscopy with 3D fluorescence imaging capability


Research and diagnosis in the life sciences depends on the information that can be found using cellular analysis. However, current microscopy techniques limit the quantity and quality of information available to researchers and clinicians

The HT-2, a microscope that displays both 3D holograms and 3D fluorescence imaging, is able to overcome these limitations through use of new and existing technology. Its abilities can enable researchers and clinicians to break into new frontiers of understanding, diagnosing, and treating disease.
HT-2 is the world’s first microscopy combining both holotomography and 3D fluorescence imaging in one unit.

The HT-2 is an update to our HT-1 microscope. The HT-1 microscope uses holotomographic technology to measure the 3D and 4D refractive indexes using a dynamic micromirror device (DMD) to add unprecedented stability and precision to the performance of the microscope. These innovations allow for quantitative, label free imaging of cells. The HT-2 has all the capabilities of the HT-1, but combines the holotomographic imaging capabilities of the original version with the added ability to perform fluorescence microscopy.

  • A three-channel LED light source

(385 nm, 470 nm, 570 nm)
Wavelengths of the LED source can be customized

  • Z-stack images with a motorized Z-drive (step resolution: 150 nm)
  • Correlative analysis in 2D, 3D and 4D with HT and fluorescence images
  • Upgradable: The HT-1 can be upgraded easily to the HT-2(fluorescence version) in the field


  • Correlative microscopy in one instrument

HT-2 provides high-quality 3D images of both holotomography and 3D fluorescence for each sample.

  • Quantitative data marked with fluorescence

HT-2 provides morphological (volume, surface area, projection area, sphericity and ellipticity), chemical (dry mass, concentration) and mechanical (cell deformability) properties of cells with 3D refractive index (RI) tomogram. Moreover, fluorescence image provides information about molecular specificity.