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Tomocube HT-X1 Holotomography

By capturing the intrinsic refractive index (RI) of cells using a low level of light intensity, Holotomography has emerged as a unique solution for live cell imaging that surpasses the compromise between obtaining high image quality and maintaining healthy cells.

Empowering your exploration. Redefining the limits.


The lastest generation of Holotomography – Tomocube HT-X1 – utilizes a low-coherence light source. This groundbreaking advancement unlocks label-free 3D and 4D quantitative live cell imaging on diverse multi-well plates with improved resolution and free of laser-induced speckle noise.

The new system works well with a wide range of biological samples, including confluent cell sheets and thick specimens. It provides not only high-content 3D visualization but also quantitative data for individual cells and their intracellular structures, such as the volume, surface area, and dry mass.


Multiplying Knowledge from 2D to 4D and Beyond

Observations of cellular morphology and activity are typically based on labeling of target molecules. These methods are invasive, affect the nature of the target molecules and potentially interfere with their biological relevance. In addition, these forms of label are not effective in providing quantitative information. The tools used to visualize labels are generally damaging to cells as often laser-based illumination is used to excite fluorochromes which in turn causes phototoxic damage to the cells.

The “holy grail” of imaging is to visualize cells without labeling to ensure the cells behave and grow normally. Holotomography (HT) does this by capturing the intrinsic light scattering properties of cellular materials using very low levels of light intensity, just enough to allow the light to pass through the cell. In doing so, the refractive index (RI) information of structures within the cell can be collected and selectively pseudo-colored to reveal the cell and its organelles.

In capturing the RI distribution, the Tomocube HT-X1 Holotomography can also provide quantitative data in 3D such as the volume, surface area, and dry mass of cells and their intracellular structures.


Correlative HT to FL


Holotomography and Fluorescence




  • High resolution, high contrast, and high sensitivity
  • Large captured fields
  • Programmable multi-well plate imaging
  • Laser autofocus for well-to-well reproducibility
  • Low noise, speckle-free imaging with no calibration step
  • Bioimaging made quantitative
  • Integrated incubation for long term timelapse
  • Integrated 4-channel FL light engine (Blue, Green, Red, Far red)
  • Correlative holotomography with 3D fluorescence
  • True multi-dimensional imaging across time, space and modality

Technical specifications

Objective Lens
  • 40 X NA 0.95 (Air)
  • Working distance 180 μm
Condenser Lens
  • NA 0.72
Lateral Resolution
(Dish/6/12/24 well)
1069/1211/1387/1695 nm
Axial Resolution

(Dish/6/12/24 well)

  • 156/161/179/205 nm
  • FOV: 218 µm x 165 µm
  • DOF: Max. 146 µm
Light Source
450 nm LED
Light Source
3 channels (R/G/B) + 1 channel (R or G or B)
  • Size (W x D x H) : 565 x 732 x 921 mm
  • Weight: 90 kg / 198 lbs
Power 100-240 V, 50/60 Hz, 5-3 A, 400 W
Temperature / Humidity 20-28 °C / 35-85 %, non-condensing

TomoStudio X



As the control software for the HT-X1, TomoStudio X is designed to operate the system as you design the experiment. TomoStudio X offers flexible workflows for multi-dimensional image acquisition of holotomography and fluorescence in multi-well imaging conditions. All the essential control and status of the system are displayed all at one glance.




  • Data acquisition
Tomocube_Label-free 3D Live Cell Imaging_Holotomography_HT-X1_Tile imaging

  • Experiment setting
Tomocube_Label-free 3D Live Cell Imaging_Holotomography_Vessel Setting



  • Timelapse sequence


User Benefits



  • 3D visualization of monolayer cell cultures and 3D organoids
  • Cellular and subcellular quantitative analysis
  • Holotomography for label-free live cell imaging
  • Correlative fluorescence for biomolecular specificity
  • Built-in incubator for long-term timelapse
  • Multi-well plates compatibility for high-throughput experiment
  • Fast, streamlined workflow for research efficiency