Superior optical performance and precision for ICSI
Nikon microscopes have played a significant role from the early days of Intracytoplasmic Sperm Injection (ICSI) and continue to contribute to the advancement of this field through superior optical performance and precision.
The ECLIPSE Ti2-U IVF provides an ultra-stable platform for performing precision manipulation procedures such as ICSI. The intermediate magnification switching function (1.5X) allows easy switching of observation magnifications without changing objectives.
Key Features
The Nikon Advanced Modulation Contrast (NAMC) technique enables high-contrast observation of colorless and transparent samples through plasticware. NAMC produces a pseudo-relief shading effect, ideal for ICSI. The direction of shading can be adjusted through 360 degrees by turning the modulator in an NAMC objective. CFI S Plan Fluor ELWD NAMC objectives are equipped with a modulator ring clamping mechanism to prevent accidental rotation of the modulator ring while rotating the correction collar.
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A sperm cell can be clearly observed in the injection pipette.
Images courtesy of: Derek Keating, B.A. and Gianpiero D. Palermo, MD, PhD., Andrology and Assisted Fertilization: Weill Cornell Medicine – Ronald O. Perelman and Claudia Cohen Center for Reproductive Medicine.
 CFI S Plan Fluor ELWD NAMC 20XC (left)CFI S Plan Fluor ELWD NAMC 40XC (right) |
 CFI Achromat NAMC 10XF (left)CFI Achromat LWD NAMC 20XF (middle) CFI Achromat LWD NAMC 40XC (right) |
This polarized light technique enables finely detailed observation of spindle bodies for oocyte assessment and the elimination of spindle damage during sperm injection.
Switching between spindle observation and NAMC is fast and easy.
The following objectives are compatible with spindle observation: CFI S Plan Fluor ELWD NAMC 20XC and CFI S Plan Fluor ELWD NAMC 40XC.
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TI2-C-SO Spindle observation system
| ECLIPSE Ti2-U IVF | |
|---|---|
| Optical System | Infinity-corrected CFI60 |
| Field Number | 22 with C-mount, 25 with F-mount |
| Intermediate Magnification Switching | Manual switching of 1.0X/1.5X |
| Output Port | 4 Manual positions Eyepiece 100%, left 100%, right 100%, eyepiece 20%/left 80% |
| Focusing Unit | Manual drive, Coarse/Fine focusing knob, Focusing stroke: 10 mm |
| Tube | TC-T-TS Binocular S tube (Field number 22) |
| Eyepiece Tube Base | TI2-T-BS S Eyepiece Tube Base Unit |
| Eyepiece | CFI 10X Eyepiece (Field number 22) |
| Pillar for transmitted illumination | TI2-D-PDI Pillar I for Dia Illumination Condenser vertical stroke: 66 mm, Backward tilting up to 25 degrees, with field diagram and refocusing mechanism |
| LED Lamphouse for Dia Illumination | TI2-D-LHLED LED Lamp House for Dia Illumination: High power LED |
| Condenser Turret | TC-C-TC Condenser Turret: 7 manual positions |
| Condenser Lens | TI-C NAMC Condenser Lens (O.D.=44 mm, NA=0.4) |
| Modules for Condenser Turret | TC-C-MN-10X NAMC 10X Module TC-C-MN-20X NAMC 20X Module TC-C-MN-40X NAMC 40X Module |
| Polarizer for NAMC Observation | TC-C-DICPNI NAMC/IMSI Polarizer |
| Stage | TC-S-SR Stage Stroke X: ±57 mm, Stroke Y: ±36.5 mm TI2-S-HL Long Handle |
| Nosepiece | TI2-N-ND DIC Sextuple Nosepiece: 6 manual positions, Simple waterproof structure TI2-N-N Sextuple Nosepiece: 6 manual positions, Simple waterproof structure |
| Spindle Observation System | TI2-C-SO Spindle Observation System |
| Adjustment Tool | C-T Centering Telescope |
| Objectives (NA/W.D.) W.D.=mm | CFI Achromat 4X (0.10/30.0) CFI Achromat NAMC 10XF (0.25/6.20) CFI Achromat LWD NAMC 20XF (0.40/3.10) CFI Achromat LWD NAMC 40XC (0.55/2.70-1.70)CFI Plan Fluor 4X (0.13/17.20) CFI S Plan Fluor ELWD NAMC 20XC (0.45/8.20-6.90) CFI S Plan Fluor ELWD NAMC 40XC (0.60/3.60-2.80) |
| Power Cord and AC Adapter | Power Cord Type BU,100-120V I-AC AC Adapter I |
| Operating Environment | Temperature: 0˚C+40˚C, Humidity: 60% RH max. (at +40˚C, no condensation), Indoor use only |
