Robust multiphoton system tailored for in vivo applications
Nikon’s multiphoton confocal microscopes, which clearly visualize fine structures deep within living organisms, have evolved even further. The AX R MP, equipped with a high speed high resolution resonant scanner, can capture dynamics that span a wide area with superior spatial and temporal resolution. In addition, a greatly expanded sample area provides the flexibility needed to observe biological samples in their natural positions and accommodates additional equipment often required for in vivo imaging.
The AX R MP maximizes image size, as well as spatial and temporal resolution, providing a uniquely powerful solution for any multiphoton experiment.
| Large Field of View | Resonant and Galvano Scanners: FOV 22 mm |
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| High-speed | Resonant Scanner: 720 fps (at 2048 x 16 pixels) |
| High-resolution | Resonant Scanner: 2K x 2K, Galvano scanner: 8K x 8K |
| High-sensitivity | Improved SNR with new detectors and electronics |
| Flexibility | Two types of stands and tilting nosepiece |
| Main body | AX-FNSP | AX-FNGP |
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| Optical system | Infinity optical system | |
| Microscope stands | AX-FNSP Single Stand | AX-FNGP Gate Stand |
| Focusing | AX-FN Focusing Nosepiece Unit Motorized coaxial coarse/fine focusing Focusing stroke: Up 13 mm/Down 2 mm*1, *2, Minimum step: 0.02 μm, Motorized escape and refocus mechanism Focal plane: 400 mm above the surface of the vibration isolated table |
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| Controls | AX-FNCTL Control Box AX-FNHC Hub Controller (For controlling Focusing Nosepiece Unit, Diascopic Illumination System, Stage Joystick, Motorized Epi-fluorescence Cube Turret, Motorized Quadrocular Tilting Tube 2 and DSC Zooming Port) |
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| Stages | AX-FNSP | AX-FNGP |
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| Adapter | AX-FNSA Stage Adapter, supporting both manual and motorized XY stages. Stage height: adjustable to 2 positions depending on sample size (400 mm/200 mm from the surface of the vibration isolated table) | |
| Stage | FN-3PS2 XY stage, Cross travel 29.5 (X) x 29.5 (Y) mm, with 2 auxiliary plates AX-FNS-E Motorized XY stage, Cross travel ±34 (X) x ±27 (Y) mm |
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| Epi-fluorescent illuminator | AX-FNSP | AX-FNGP |
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| Illumination unit | NI-FLEI-2 Epi-fluorescence attachment | |
| Light source | D-LEDI Fluorescent LED Illumination System | |
| Filter cube turret | 6 mountable filter cubes, shutter function
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| Photostimulation device | AX-FNBPU Stimulation Back Port, 6 mountable filter cubes, Fluorescence imaging and simultaneous stimulation imaging can be switched | |
| Diascopic illuminator | AX-FNSP | AX-FNGP |
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| Illumination unit | AX-FNDIA Diascopic Unit 4 filter slider attachable, Condenser holder stroke: Up 2.5 mm/Down 1.8 mm, NI-PT Polarizer Turret mountable |
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| Light source | Halogen Lamp (12V100W)
High Luminescence White LED Illuminator
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| Shutter | NI-SH-E Motorized Shutter | — |
| Condenser | FN-C LWD condenser, O.D. 8.2 mm, NA: 0.78 | — |
| Polarizer Turret | NI-PT Polarizer Turret, Visible or infrared polarizer attachable | — |
| AX-FNSP | AX-FNGP | |
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| Tubes | Pupillary distance: 50-75 mm, Inclination angle: 15-35 degrees, Eyepiece/Upper port/Rear port: 100/0/0, 0/100/0, 0/0/100 via DSC Zooming Port
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| Eyepieces (F.O.V. (mm)) | CFI 10X (22), CFI 12.5X (16), CFI 15X (14.5), CFI UW 10X (25) | |
| Photodetector | AX-NEU Non-descanned EPI Upright Detector | |
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| Observation methods | Brightfield, Epi-fluorescence, DIC, IR-DIC | |
| Power consumption | 100W | |
| Weight (approx.) | 66 kg (fully motorized fluorescence system, with diascopic illuminator) | 66 kg (fully motorized fluorescence system) |
| Scan head | AX R MP |
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| Type | AX-SHRM AX R MP Scan Head & Controller |
| FOV | φ22 mm |
| Standard image acquisition | Galvano scanner Pixel size: max. 8192 x 8192 pixels Scanning speed: max. 240 fps (512 x 16 pixels), 10 fps (512 x 512 pixels) Zoom: 1-1000X continuously variable Supports line scanning, bi-direction scanning and averaging (line and rolling) |
| High-speed image acquisition | Resonant scanner Pixel size: max. 2048 x 2048 pixels Scanning speed: max. 720 fps (2048 x 16 pixels for 2K, 1024 x 16 pixels for 1K), 30 fps (2048 x 512 pixels for 2K, 1024 x 512 pixels for 1K) Supports line scanning, bi-direction scanning and averaging (line and rolling) Enables simultaneous acquisition of a maximum of 5 channels (with the inclusion of a diascopic detector channel) Equipped with Denoise.ai noise reduction function |
| IR laser wavelength range | 700-1080 nm (1080 system), 820-1300 nm (1300 system) |
| Dichroic mirror | Position: 6 |
| Pinhole | 6-153 µm variable |
| Input/output port | 2 laser input ports 2 signal output ports |
| Laser for multiphoton microscopy | AX R MP |
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| Single 1080 system | Mai Tai HP/eHP DeepSee, Chameleon Vision II, Axon 920 |
| Dual 1080 system | Chameleon Vision II + Axon 920, Axon 920 + Axon 1064 |
| Single 1300 system | InSight X3, Chameleon Discovery NX |
| Dual 1300 system | InSight X3 Dual Option, Chameleon Discovery NX, Chameleon Discovery NX + Axon 920 |
| Incident optics | 700-1080 nm (1080 system), 820-1300 nm (1300 system), auto alignment |
| Modulation | Method: AOM (Acousto-Optic Modulator) device Control: power control, return mask, ROI exposure control |
| Laser for confocal microscopy (option) | AX R MP |
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| LUA-S4 laser unit | 405 nm, 488 nm, 561 nm and 640 nm lasers are installed |
| LUA-S5 laser unit | 405 nm, 488 nm, 561 nm, 594 nm and 640 nm lasers are installed |
| LUA-S6 laser unit | 405 nm, 445 nm, 488 nm, 515 nm, 561 nm and 640 nm lasers are installed |
| NDD for multiphoton microscopy | AX R MP |
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| NDD EPI unit AX-NEU (for Ti2-E) and AX-NEI (for AX-FNSP/FNGP) | Detectable wavelength range: 400-650 nm (1080 system), 400-750 nm (1300 system)
Detectors: 2 GaAsP PMTs (4 GaAsP PMTs, or 3 GaAsP PMTs + 1 multi-alkali PMT are possible by adding options) |
| Visible stimulation/IR imaging (option) | AX R MP |
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| Opti-Microscan Photostimulator (for AX-FNSP/AX-FNGP) | Stimulation wavelength: 405 nm, 488 nm, 561 nm; Excitation wavelength for imaging: 800-1080 nm (1080 system), 820-1080 nm (1300 system) Stimulation speed: Max. 1 ms (point stimulation), Max. 20 μs/pixel (ROI stimulation) Stimulation modes: simultaneous, sequential, manual Stimulation area: square inscribed within a 22 mm-diameter circle, stimulation ROI: arbitrary pattern, no number limit |
| Diascopic detector (option) | AX R MP |
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| AX-DUT-MP (for AX-FNSP/Ti2-E)*6 | Detectable wavelength range: 400-920 nm Detector: Multi-alkali PMT |
| Detector for confocal microscopy (option) | AX R MP |
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| DUX-VB detector unit | Detectable wavelength range: 400-650 nm (with IR laser), 400-750 nm (with visible laser); Detection width: 10 nm to 320 nm Maximum pixel size: 8192 x 8192 (with galvano scanner) Wavelength resolution: 5 nm, wavelength range variable in 1 nm steps Compatible with galvano and resonant scanners2 or 4 channels (Multi-alkali PMT or GaAsP PMT options) |
| DUX-ST detector unit | Detectable wavelength range: 400-750 nm 2 or 4 channels (Multi-alkali PMT or GaAsP PMT options) |
| Option | AX R MP |
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| Motorized XYZ | Motorized XY stage (for AX-FNSP/FNGP/Ti2-E), High-speed piezo Z stage (for Ti2-E), High-speed piezo objective-positioning system (for AX-FNSP/FNGP) |
| Nosepiece for AX-FNSP/FNGP | AX-FNTN-H CFI75 single tilting nosepiece*4 |
| Software | AX R MP |
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| Acquisition/analysis | NIS-Elements C or NIS-Elements C-ER imaging software |
| Display/image generation | 2D analysis, 3D volume rendering/orthogonal, 4D analysis, spectral unmixing |
| Image format | JP2, JPG, TIFF, BMP, GIF, PNG, ND2, JFF, JTF, AVI, ICS/IDS |
| Application | FRAP, FLIP, FRET(option), photoactivation, 3D time-lapse imaging, multipoint time-lapse imaging, colocalization |
| Control computer | AX R MP |
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| OS | Windows®10 Pro 64 bit |
| AX R MP | |
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| Visible stimulation/IR imaging (option) | Stimulation wavelength: 405 nm, 488 nm, 561 nm Excitation wavelength for Imaging: 800-1080 nm (1080 system), 820-1080 nm (1300 system) |
| Compatible microscopes | Dedicated AX-FNSP/AX-GNGP motorized upright microscope system, ECLIPSE Ti2-E motorized inverted microscope |
| Z step | AX-FNSP/FNGP: 0.02 µm, Ti2-E: 0.02 µm |
| Recommended installation conditions | Temperature 20 – 25˚C, ± 1˚C, air conditioning at all hours Humidity 60% RH or less (no condensation) |
*1 Based on the focus position
*2 Software controlled value
*3 DIC prism slider cannot be attached
*4 FOV 12, Usable objectives: CFI75 LWD 16X W, CFI75 Apochromat LWD 20XC W, CFI75 Apochromat 25XC W, CFI75 Apochromat 25XC W 1300
*5 Cannot be used with diascopic illlumination. The FN-MN-H cannot be used with diascopic illumination only when the 400 μm objective piezo positioner (PI) is attached.
*6 Cannot be mounted on AX-FNGP
Objectives for Multiphoton Microscopy
Bright images, high quality
Nikon offers a full lineup of high NA objectives for multiphoton imaging that provide chromatic aberration correction up to the near-infrared range. These objectives are optimized for deep imaging and provide bright images over the entire field of view.
Nikon’s exclusive Nano Crystal Coat is an antireflective coating consisting of ultrafine crystalline particles. This forms a coarse structure that enables lower refractive indices, facilitating the passage of light through the lens rather than reflecting it, thus providing superior light transmission.
Water immersion objectives
The water immersion range of objectives provides the highest NA, high flexibility, and compatibility with upright, inverted, multiphoton or confocal instruments. The CFI75 models are dedicated to upright microscopes and imaging at greater depths (2~3 mm).
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 CFI Apochromat Lambda S 40XC WI
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 CFI Apochromat LWD Lambda S 20XC WI
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 CFI75 LWD 16X W
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 CFI Apochromat NIR 40X W
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 CFI Apochromat NIR 60X W
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Silicone immersion objectives
Silicone oil closely matches the refractive index of live cells, thereby minimizing spherical aberration and providing brighter, higher-resolution images in deep imaging.
 CFI Plan Apochromat Lambda S 25XC Sil
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 CFI Plan Apochromat Lambda S 40XC Sil
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Glycerol objectives
The glycerol range of objectives is dedicated to extreme depth (5~8 mm) observation of cleared samples. The major benefit of these lenses is their ability to correct for changes in refractive indices and compensate for aberrations that often occur in large sample imaging.
 CFI Plan Apochromat 10XC Glyc
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 CFI90 20XC Glyc
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Multi-immersion objectives
A multi-immersion objective can be used for oil immersion, glycerin immersion, and water immersion.
 CFI Plan Fluor 20XC MI
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Dry objectives
This dry objective achieves uniform brightness up to the edge of the image, chromatic aberration correction from 405 nm to 850 nm, and high resolution. It provides wide area acquisition and high throughput.
 CFI Plan Apochromat Lambda D 10X
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