NovaFLIM

Fluorescence Lifetime Imaging Analysis Software

Imaging analysis with Luminosa, MicroTime 200, and LSM Upgrade Kits
Fast analysis of FLIM, FLIM-FRET, and anisotropy data, based on GPU-accelerated algorithms
Efficient batch analysis of z-stacks, time-lapse series and stitched images
Advanced, flexible ROI handling
Reproducible ROI selection incorporating histograms of fitted parameters

Advanced analysis of images at your fingertips with the click of a button

Quality and precision you can trust

Multi-faceted view of your FLIM data by combining multi-exponential fitting, phasor plots, and pattern matching
Quantitative, robust, and reproducible ROI selection via 1D and 2D histograms or from phasor plot

Save time and simply focus on your samples

Seamlessly integrated with Luminosa
High speed thanks to GPU-based algorithms
Minimal user interaction required
Efficient analysis of series measurements, including batch analysis and parameter plots of multiple-ROIs

Advanced flexibility

Simultaneous batch analysis for FLIM, FLIM-FRET, anisotropy for user defined channels
Many data export options for graphs and images (Bitmap, PNG, OME TIFF, Scalable Vector Graphics)

PC system requirements
Licence type	Software licence, available for either 1, 2, or 4 years
Data formats	Files acquired with Luminosa Files acquired with SymPhoTime 64 (version 2.5 from March 2020 or higher) using a MicroTime 200, MicroTime 100, or LSM Upgrade Kit
	Minimum	Recommended
Operating system	Win10 64-bit	Win10 / Win11 64-bit
CPU	Intel Core i3-8100 or AMD Ryzen 3 1300X	Intel Core i7-13700 or AMD Ryzen 7 5700X
RAM	8 GB	32 GB
GPU	AMD Radeon RX550/550 Series or Nvidia GeForce GT 1030	AMD Radeon RX 6700 XT or Nvidia GeForce RTX 3060
Screen resolution	QHD (2560 × 1440)	4K (3840 × 2160)

NovaConvert – included software to convert *.spqr files and *.ptu files acquired with SymPhoTime 64 (version 2.3 from July 2017 or higher) to be analyzed in NovaFLIM.

NovaConvert screenshot: defining detection channels for converting *.ptu file acquired with SPT64 to *.spqr file for analysis with NovaFLIM

All Information given here is reliable to our best knowledge. However, no responsibility is assumed for possible inaccuracies or omissions. Specifications and external appearances are subject to change without notice.

	Luminosa	NovaFLIM
InstaFLIM Fast GPU accelerated analysis with minimal user interaction 4 different FLIM contrast options: Fast lifetime, multi-exponential decay fit, phasor plot, and pattern matching Suggestion for FLIM species separation Suggested parameters can be fine-tuned
Phasor plot for marker multiplexing
Multi-frame analysis
Batch analysis of series measurements (time-lapse,z-stacks, tiling, …)
Advanced ROI handling, multi-ROIs, import of external ROIs
Parameter plots of fitted parameters over multiple ROIs
Representation of the image information as user defined 1D and 2D histograms of fitted parameters
ROI selection via parameter histograms
User configured analyses allowing for FLIM, FLIM-FRET, steady-state FRET and anisotropy imaging
Extended export options of graphs and images
Image Scanning Microscopy-FLIM (Resolution enhancement via a pixel reassigned image available in combination with PDA-23 Add-on)
Re-assignement vectors calculation from image