Okolab – Bold Line 3 Stagetop Incubator

BL3 is the first microscope incubation solution to provide closed-loop feedback control at the sample level for temperature, humidity, and gas composition, ensuring higher experimental reliability, lower gas consumption and error-proof workflows.

The stagetop chamber guarantees superior environmental control around your sample. Modular inserts adapt to multiple stage geometries.

Models available for all XY stages/piezos on the market, including upright microscopes.
Wireless heated sliding lid – low profile for Koehler compatibility.
Can natively accommodate multi-well plates without additional inserts.
Universal Sample Holders can be combined with a simple adapter for use in the XY stage/piezo without incubation.
In-chamber sensor for complete sample feedback provides an unprecedented level of accuracy and repeatability – ensures reliable environment where it matters most.
Perfusion holes for inlet and outlet of microfluidic tubes.

The BL3 Controllers are designed to deliver precise and reliable environmental control inside the cage incubator, ensuring optimal conditions for long-term live-cell imaging.

Each configuration combines advanced temperature regulation with optional closed-loop CO₂ and O₂ gas control, allowing users to choose the level of environmental management that best suits their application.

Available Product Bundle

ONLY T

Temperature range: from 3°C above ambient to 45°C
Temperature accuracy: ± 0.1°C
Low energy consumption thanks to warm air recirculation
One-touch control of all environmental parameters from a single intuitive touchscreen panel, powered by intelligent software that automatically regulates the system

T-H-CO2 CONTROLLER

Delivers combined control of temperature and CO₂ concentration for standard live-cell imaging applications.

Above temperature specifications
CO₂ range: 0 – 20 %
CO₂ accuracy: ± 0.1 %
CO₂ sensor: NDIR dual-wavelength, 10-year lifetime
Air pump flow rate: 0.05 – 0.4 l/min (up to 1l/min with compressed air tank)

T-H-CO2-HYPOXIA CONTROLLER

Adds oxygen control for hypoxia, anoxia, and normoxia studies, expanding the system’s experimental versatility

CO₂ range: 0 – 20 %, accuracy ± 0.1 %
O₂ range: 0 – 21 %, accuracy ± 0.1 % at 5 % O₂
O₂ sensor: Optical, 5-year lifetime
Air pump flow rate: 0.05 – 0.4 l/min (up to 1l/min with compressed air tank)

T-H-CO2-HYPEROXIA CONTROLLER

Provides full oxygen control for advanced anoxia, hypoxia, and hyperoxia applications.

CO₂ range: 0 – 20 %, accuracy ± 0.1 %
O₂ range: 0 – 95 %, accuracy ± 0.1 %
Gas mixing via digital mass flow controllers for N₂, O₂, CO₂
Air pump flow rate (when using Air/CO2 Mode) : 0.2 – 0.4 l/min (factory set 0.4 l/min)

Independent humidity module keeps chamber relative humidity stable and protects samples during long imaging sessions. Digital humidity sensor provides active feedback.

Features

Controls relative humidity inside the stagetop chamber in the range 50% to 95% at 37°C.
Choose the preferred humidity level in 1% increments easily, using OKO-TOUCH interface.
External humidity module – NO water moat. Keeps water safely away from your microscope.
Water heater humidifies the gas mixture.
Tube heater prevents condensation and maintains humidity level between water heater and chamber.

One-touch control of all environmental parameters from a single intuitive touchscreen panel, powered by intelligent software that automatically regulates the system.