Holotomographic microscopy HT 2

The HT-2 series opens a new era of 3D correlative imaging, combining the holotomography and fluorescence methods.
The HT-2 allows the viewing of a 3D RI tomographic image of a living cell with minimal damage, while at the same time targeting consumer interest by allowing for fluorescence labeling in the vary same cell.

 

HT-2 : Holotomographic microscopy with 3D fluorescence imaging capability

 

Research and diagnosis in the life sciences depends on the information that can be found using cellular analysis. However, current microscopy techniques limit the quantity and quality of information available to researchers and clinicians

The HT-2, a microscope that displays both 3D holograms and 3D fluorescence imaging, is able to overcome these limitations through use of new and existing technology. Its abilities can enable researchers and clinicians to break into new frontiers of understanding, diagnosing, and treating disease.
HT-2 is the world’s first microscopy combining both holotomography and 3D fluorescence imaging in one unit.

The HT-2 is an update to our HT-1 microscope. The HT-1 microscope uses holotomographic technology to measure the 3D and 4D refractive indexes using a dynamic micromirror device (DMD) to add unprecedented stability and precision to the performance of the microscope. These innovations allow for quantitative, label free imaging of cells. The HT-2 has all the capabilities of the HT-1, but combines the holotomographic imaging capabilities of the original version with the added ability to perform fluorescence microscopy.

Holotomographic Microscopy – HT 1

HT-1 is a new microscope designed to let researchers view live cell using holotomograhpy technology. One of the world’s first microscopes of its kind, the HT-1 delivers non-invasive, real-time quantitative data with the highest resolution on the market today.

Holotomographic Microscopy – HT 1

Revolutionary holotomographic (3D holographic) microscopy opens new era for label-free live cell imaging

Cellular analysis plays a crucial role in a wide variety of research and diagnostic activities in the life science. However, the information available to researchers and clinicians is limited by current microscopy techniques. An innovative new tool – Holotomographic microscopy – can overcome many of these limitations and open new vistas for researchers and clinicians to understand, diagnose and treat human diseases.

Holotomographic Microscopy – New era of microscopy Tomocube’s holotomography series utilize optical diffraction tomography (ODT), which enables users to quantitatively and noninvasively investigate biological cells and thin tissues. ODT reconstructs the 3D refractive index (RI) distributions of live cells and by doing so, provides structural and chemical information about the cell including dry mass, morphology, and dynamics of the cellular membrane.

TomoStudio™, Software platform for 2D/3D/4D image analysis

TomoStudio™, HT-1 operating SW, controls the system and visualizes the captured image in various ways. This flexible user interface provides fast imaging capability and 2D/3D/4D visualization of the cellular image based on 3D RI distributions of the cells and tissues. follow this link. TomoStudio™

Smart technology for holotomography

HT-1 use holotomography technology which measures the 3D and 4D refractive index (RI) tomograms. This new technology enables quantitative, label-free measurements of live, unstained cells and tissue samples. With the dynamic micromirror device (DMD), we have eliminated moving parts from light path while image-capturing and has added unprecedented stability and precision on the outstanding performance of our product. If you want to get more information about our technology, follow this link. Technology

LUNA Phase Only Spatial Light Modulator (Reflective)

The LUNA phase only Spatial Light Modulator (SLM) consists of a driver unit with standard digital video interface (DisplayPort) and a phase only LCOS (Liquid Crystal on Silicon) microdisplay with full HD resolution (1920 x 1080 pixel) and 4.5 µm pixel pitch leading to an active area diagonal of 0.39” with an aspect ratio of 16:9.

The LUNA Spatial Light Modulator is our most compact SLM platform for integration into small sized or even portable solutions.

The LUNA phase only Spatial Light Modulator (SLM) consists of a driver unit with standard digital video interface (DisplayPort) and a phase only LCOS (Liquid Crystal on Silicon) microdisplay with full HD resolution (1920 x 1080 pixel) and 4.5 µm pixel pitch leading to an active area diagonal of 0.39” with an aspect ratio of 16:9.

The driver ASIC is embedded in the LCOS microdisplay itself. This saves board space which enables a very compact driver and makes integration more convenient. The standard driver box has a size of only 84.4 x 47 x 28.8 mm. The display can even accept video data input via a 4-lane MIPI DSI. This brings phase only Spatial Light Modulator technology to a new level of potential for industrial implementations.

The LUNA SLM usees fast full digital addressing which assures high reliability and a compact driver unit. A certain gray level represents a defined average voltage across the LC cell. This voltage leads to a variable tilt of the LC molecules due to their electrical anisotropy. As LC molecules also show optical anisotropy this tilt changes the refractive index of the LC molecules (for incident polarization along the long display axis) which causes a modified optical path length within the LC cell. The addressed gray level is converted into a phase level.

The LUNA driver unit is equipped with an USB interface for changing the voltage vs. phase level distribution (gamma control) and dynamic range (voltage across the LC cell) in order to calibrate the SLM for different wavelengths.

The LUNA SLM is a plug & play phase modulator and can be addressed with phase functions via standard graphics cards as extended monitor device. Addressing can be done using the supplied Pattern Generator software, the HOLOEYE Slideshow Player software or standard image viewer software. HOLOEYE also provides an SLM Display Software Development Kit (SDK) which provides APIs (Application Programming Interface) for different programming languages.

LUNA Spatial Light Modulator – Microdisplay Features
Display Type: Reflective LCOS (Phase Only)
Resolution: 1920 x 1080
Pixel Pitch: 4.5 µm
Fill Factor: 91 %
Active Area 8.64 x 4.86 mm (0.39″ Diagonal)
Addressing 8 Bit (256 Phase Levels)
Signal Formats DisplayPort – HDTV Resolution
Input Frame Rate 60 Hz

LUNA Phase Only Spatial Light Modulator Versions

LUNA LCOS SLM Wavelengths Ranges

HOLOEYE offers different phase display versions which can be driven with the LUNA driver unit. The different display versions are optimized for the use at different wavelength ranges. Currently we offer a VIS version (420 – 650 nm) and a version for the telecommunication waveband around 1550 nm (1400-1700 nm).

Version Wavelength Range Reflectivity Max. Phase Shift
LUNA-VIS-111 420 – 650 nm 61-67% 4.3 π @ 450 nm
3.2 π @ 520 nm
2.4 π @ 635 nm
LUNA-TELCO-112 1400 – 1700 nm 70 % 2.3 π @ 1550 nm

LUNA Spatial Light Modulator Kit – Contents:

 

LUNA Spatial Light Modulator – Scope of Supply
LUNA phase display (1920 x 1080 pixel) incl. flex cable
LUNA driver unit
USB Cable
DisplayPort / Mini DisplayPort cable
Magnetic display mount & display adaptor
Screw adaptor M4 to ½”
Safety instructions & quick-start guide
USB flash drive with documentations, detailed manual, Configuration Manager and SLM software

LUNA Phase Modulator – Software Features:

he LUNA phase only spatial light modulator devices can simply be addressed like an external monitor using the standard DisplayPort interface of the graphics card. No additional software or dedicated hardware is needed to operate the SLM.

The device is delivered with a Configuration Manager software. The Configuration Manager can be used to change geometrical settings, brightness, contrast and the electrooptical response by applying a new gamma curve or another digital drive scheme. For these advanced calibrations the device uses an USB connection to a virtual COM port of the computer.

In addition a HOLOEYE SLM Pattern Generator software is provided with the SLM:

  • Computation of computer generated holograms (CGH) from user defined images
  • generation of SLM signals representing basic optical functions such as lenses, gratings, axicon and vortex functions
  • superposition of CGH’s with basic optical functions to combine functionalities

Besides that a special SLM Slideshow Player Software comes with the device. Also an SLM Display SDK is available for download which provides APIs (Application Programming Interface) for different programming languages to show images and data/ phase arrays directly on a HOLOEYE Spatial Light Modulator:

  • National Instruments LabVIEW™ 8.6 and later
  • MathWorks MATLAB® R2009b and later
  • Octave 5.2
  • Python™ 2.7 and 3.x
  • C or C++ compiler (e. g. Microsoft Visual C++ Compiler)

Driver Dimensions (Unit: mm):

LUNA LCOS Spatial Light Modulator Driver Dimensions

Display Dimensions (Unit: mm):

The current LUNA microdisplay package has a size of only 25.7 x 12.6 x 3.4 mm. For more convienient mounting we deliver it with a display mounting adaptor.

LUNA LCOS Spatial Light Modulator Display Adaptor

 

LETO-3 Phase Only Spatial Light Modulator

The LETO-3 phase only Spatial Light Modulator is based on a reflective LCOS microdisplay with full HD (1920 x 1080 pixel ) resolution. With a pixel pitch of 6.4 µm and a small interpixel gap of 0.2 µm the LETO-3 SLM provides a high fill factor of 93%.

The LETO-3 Spatial Light Modulator offers a reflectivity between 62% up to 75% (dependent on wavelength and version). The device offers diffraction efficiencies of more than 80% (16 level blazed grating) which leads to a total light efficiency of ~ 60%.

The LETO-3 SLM driver is prepared to work in color-field-sequential (CFS) mode e.g. with color-switchable LASER or LED lighting and the device features an LED-connector which can be used to synchronize the light source with the device. The driver features 4 slots with preconfigured CFS configurations for different environments which can be slected using the Configuration Manager Software.

 

 

Versions

HOLOEYE offers three different LETO-3 Spatial Light Modulator versions which are optimized for the use at different wavelength ranges / for different applications. One SLM version is optimized for the visible range, one version for the near IR range up to 1100 nm and a third version is optimized for fast response (180 Hz) and use for color-field-sequential (CFS) operation in the visible range with color-switchable RGB Laser.

LETO-3 Spatial Light Modulator Wavelength Ranges

QNW – Temperature-Controlled Cuvette Holder for UV/Vis Spectrophotometers

The t2 Sport™ is a Peltier-Temperature controlled cuvette holder with two optical ports for absorbance. It has a short, compact structure that will fit into nearly any UV/Vis spectrophotometer.

t2 Sport™: Temperature Controlled Cuvette Holder for UV/Vis Spectrophotometers

The device is mounted on a post to allow adjustment of the cuvette to the optimal height. On ordering, the user selects the cuvette holder z-height of 8.5 or 15 mm. With variable-speed magnetic stirring, the t2 Sport™ is built to maintain uniform temperature within the cuvette. A dry gas purge system directs dry gas to the surfaces of the cuvette to minimize condensation when working below the dew point temperature.

The t2 Sport™ package includes a matched and calibrated TC 1 Temperature Controller, and provides rapid, precise temperatures in the range of -15 °C to +110 °C with a precision of ±0.02 °C. While lower temperatures may be readily achieved, it is important to avoid the risk of condensation on cuvette surfaces. The TC 1 controller has a probe input that accepts a Series 400 or Series 500 thermistor probe for independent measurement of the sample temperature.

Versions of the t2 Sport™ are available for mounting in a variety of different spectrophotometers. The device can be easily adapted to others as needed. For more information on an individual adaptation, click on a version below.

Force/Torque Sensor

A device that measures the outputting forces and torques from all three Cartesian coordinates (x, y, and z). A six-axis force/torque transducer is also known as a multi-axis force/torque transducer, multi-axis load cell, F/T sensor, or six-axis load cell.

Measure all six components of force and torque in a compact, rugged transducer.

The ATI Multi-Axis Force/Torque Sensor system measures all six components of force and torque. The system consists of a transducer, shielded high-flex cable, and intelligent data acquisition system, Ethernet/DeviceNet interface or F/T controller. Force/Torque sensors are used throughout industry for product testing, robotic assembly, grinding and polishing. In research, our sensors are used in robotic surgery, haptics, rehabilitation, neurology and many others applications.

ATI offers two types of sensing technologies:

• Force/Torque Sensor
• Capacitive F/T

Gamma: Multi Axis Force Torque Transducer

The ATI Multi-Axis Force/Torque Sensor system measures all six components of force and torque. It consists of a transducer, interface electronics and cabling.

Transducer
The compact and rugged monolithic transducer uses silicon strain gages to sense forces. The transducer’s silicon strain gages provide high noise immunity and allow high overload protection, which is standard on all models.

Built-In Capabilities

The Net F/T, DAQ F/T, Controller F/T, and TWE F/T each provide a variety of powerful functions:

• Tool transformations translate and/or rotate the F/T reference frame.
• Demo software allows configuration and basic data logging capabilities.
• Biasing provides a convenient way to offset tool weight.
• Increased system throughput is possible by reducing the number of axes of output. (Not applicable to the Net F/T or TWE F/T.)
• Threshold detection eases integration into industrial applications (Net    F/T and Controller F/T only).
• Integral temperature compensation insures accuracy over a wide temperature range.

New Technology – Capacitive F/T

ATI also offers the Capacitive F/T, a Six-Axis Force/Torque Sensor that utilizes capacitive technology in a simple structure, providing a low-cost solution. The Capacitive F/T includes integral overload protection and is IP65-rated for protection against dust and water spray. The sensor is easily connected to computer systems using the optional USB or Ethernet conversion cables. More information here.

PLUTO-2 Phase Only Spatial Light Modulator (Reflective)

The PLUTO-2 phase only Spatial Light Modulator (SLM) consists of a driver unit with standard digital video interface (HDMI) and a phase only LCOS (Liquid Crystal on Silicon) microdisplay with full HD resolution (1920 x 1080 pixel) and 8 µm pixel pitch leading to an active area diagonal of 0.7” with aspect ratio of 16:9.

The PLUTO-2 SLM is a plug & play phase modulator device and can be addressed with phase functions via standard graphics cards as an extended monitor device. The green color channel of the video signal is used for addressing 8 bit gray level patterns (the SLMs native resolution need to be addressed). Addressing can be done using the supplied Pattern Generator software or the SLM Slideshow Player software or standard image viewer software. HOLOEYE also provides an SLM Display Software Development Kit (SDK) which provides APIs (Application Programming Interface) for different programming languages.

HOLOEYE offers different display versions that can be driven with the PLUTO driver unit. The different panel versions are optimized for the use at different wavelength ranges and different applications. All versions use fast full digital addressing which assures high reliability and a compact driver unit as basically only two voltages need to be generated.

The PLUTO-2 driver uses an HDMI interface for addressing phase functions and an USB connection to communicate with the driver (to changing the voltage vs. gray level distribution (gamma control) and dynamic range (voltage across the LC cell) in order to calibrate the SLM for different wavelengths). Besides this the driver features a trigger sync output to synchronize the device with external devices.

The PLUTO-2 driver also features a dual-core ARM® Cortex™-A9 processor which also includes on-chip memory. This enables the user to program additional functionality which is directly processed on the PLUTO-2 device (e.g. a slideshow from images which are loaded from USB flash or from internal memory). The dual-core system runs an embedded Linux™ SMP operating system and includes a library which provides full control and supervision of the display and driver board. The PLUTO-2 provides access through Serial and Ethernet-over- USB2 (RNDIS) interfaces and can be programmed using standard Ubuntu™ cross compile GCC toolchain.

The standard PLUTO displays show a reflectivity of approx. 65%-95% (dependent on version) and diffraction efficiencies of more than 80%. Thereby a total light efficiency of more than 50% per addressable diffractive device is possible.

PLUTO Spatial Light Modulator – Microdisplay Features
Display Type: Reflective LCOS (Phase Only)
Resolution: 1920 x 1080
Pixel Pitch: 8.0 µm
Fill Factor: 93 %
Active Area 15.36 x 8.64 mm (0.7″ Diagonal)
Addressing 8 Bit (256 Grey Levels)
Signal Formats DVI – HDTV Resolution
Frame Rate 60 Hz

Download PDF: NEW! PLUTO-VIS-020 Phase Only Modulator (530-640nm)
Download PDF: NEW! PLUTO-NIRO-020 Phase Only Modulator (1100-1400nm)
Download PDF: Brochure Spatial Light Modulators

LC 2012 Spatial Light Modulator (transmissive)

The LC 2012 Spatial Light Modulator is based on a transmissive Liquid Crystal (LC) microdisplay with 1024 x 768 pixel resolution. The SLM provides a phase shift of about 2 π at 450 nm, about 1.8 π at 532 nm and around 1 π at 800 nm. The microdisplay and drive electronics are packaged into a compact box for easy integration into optical setups. The LC 2012 is addressed using a standard HDMI interface and brigtness and contrast settings can be performed using an USB interface. The LC 2012 Spatial Light Modulator can be used for phase (phase mostly) and amplitude modulation applications dependent on the configuration.

LC 2012 Spatial Light Modulator – Microdisplay Features
Display Type: Transmissive LC
Resolution: 1024 x 768
Pixel Pitch: 36 µm
Fill Factor: 58 %
Active Area 36.9 x 27.6 mm (1.8” Diagonal)
Addressing 8 Bit (256 Grey Levels)
Signal Formats HDMI – XGA Resolution
Frame Rate 60 Hz

Download PDF: Brochure Spatial Light Modulators

+1/-1 order Phase Masks (FBG)

Phase masks are the production tool used to write gratings in fibers and waveguides. Ibsen Phase masks incorporate unbeatable, interferometric (holographic) patterning technology into a production friendly Phase mask.

– for easy and cost-effective fabrication of Bragg gratings

Ibsen’s +1/-1 order principle Phase masks ensure quality and facilitate production in the telecommunications, sensor and laser industries. In the production of fiber and waveguide Bragg gratings, the Phase mask is illuminated by a high-power UV laser, typically an excimer laser or frequency-doubled gas laser. Self-interference between the diffracted orders produces the required pitch in the fiber or waveguide.