Archives: Product

Streamlined multiphoton confocal for deeper, faster multiphoton imaging.

Nikon’s A1 MP+ is a streamlined version of the unique A1R MP+ multiphoton imaging system developed for simplified, cost-effective multiphoton imaging.

Enables single molecule visualization, allowing dynamic observation and functional analyses of both in vitro and living cells

Illuminator for TIRF Applications
Newly developed motorized laser TIRF illumination unit allows laser incident angle adjustment, shutter control and switching to widefield fluorescence excitation with the control pad or NIS-Elements software. The laser incident angle can be stored with a single touch of the control pad button. Stored laser incident angles can be easily reproduced. This enables alternate time-lapse recording between fluorescence and multi-wavelength TIRF images.

Modular illumination system with ultimate flexibility and expandability

The Nikon Ti-LAPP system provides modular illuminators for total internal reflection fluorescence (TIRF),photoactivation/conversion, photobleaching and epi-fluorescence.
Each module can be flexibly combined to build microscope systems that are optimized for individual research needs. For example, multiple TIRF modules can be incorporated into a single microscope for anisotropy experiments and fast, multi-angle TIRF imaging. Combined with the Ti’s stratum structure, up to five illumination modules can be incorporated into a single microscope (e.g. two TIRFs, a FRAP, a DMD and an Epi-FL module can all be integrated into one Ti).

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Ti-LAPP Modular Illumination System

All-new super-resolution microscope featuring acquisition speeds ten times faster than conventional N-STORM systems.

Nikon’s Next Generation N-STORM Super-Resolution Microscope System.
With ten times faster acquisition speeds compared to conventional N-STORM, N-STORM 4.0 enables imaging of live cells with nanoscale resolution.

Nikon has established a leading position in the field of super resolution microscopy since the release of N-STORM in 2010. STORM, or Stochastic Optical Reconstruction Microscopy is a super-resolution technique developed by Dr. Xiaowei Zhuang, a Howard Hughes Investigator at Harvard University. The technology localizes temporally isolated fluorescent molecules to reconstruct images with nanoscale resolution (~10 times greater resolution compared to conventional light microscopy). N-STORM 4.0 is the next step in the evolution of STORM imaging, enabling STORM imaging of dynamic processes in live cells.

N-STORM 4.0 utilizes an improved laser excitation design and a sCMOS camera that dramatically improves acquisition rates.

There is a rapidly growing interest in super-resolution microscopy as evidenced by the recent 2014 Nobel Prize in Chemistry awarded to the field of super-resolution microscopy. There is also an increasing need to expand these techniques to broader applications like live-cell imaging. Recent studies show that localization-based super-resolution imaging of live cells can provide unprecedented insights into the nanoscale dynamics of intracellular structures such as focal adhesions1, transferrin clusters in clathrin-coated pits2, and organelles including mitochondria and endoplasmic reticulum3.

N-STORM 4.0 utilizes an improved laser excitation design and a sCMOS camera to improve acquisition rates from minutes to seconds while gathering incredibly high molecule counts. In addition, the new system offers expanded flexibility in the imaging field of view, allowing users to choose the best suited mode for their application, whether it be high-speed imaging of live cells or gathering large fields of view of fixed cells.

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N-STORM 4.0 utilizes an improved laser excitation design and a sCMOS camera that dramatically improves acquisition rates.

1 Shroff, H. et al., 2008, Nature Methods, 5 (5), p417-423.
2 Jones, S.A. et al., 2011, Nature Methods, 8 (6), p499-505.
3 Shim, S-H. et al., 2012, Proc. Natl. Acad. Sci., 109, p13978-13983

All-new personal structured illumination super-resolution system for the individual lab.

Introducing an all-new super-resolution system for the individual lab. Achieve 2x resolution at half the price!
Utilizing structured illumination microscopy (SIM) technology, the all-new N-SIM E realizes double the spatial resolution of conventional optical microscopes (to approximately 115 nm). N-SIM E is a streamlined, affordable super-resolution system supporting only essential, commonly used excitation wavelengths and imaging modes, making it an obvious choice for individual labs.

Structured illumination super-resolution microscope delivering twice the resolution of traditional diffraction limited microscopes.

Super-resolution microscope visualizing cellular structures and molecular activity at resolutions never before achieved by conventional light microscopy.
Nikon’s N-SIM microscopy system can produce twice the resolution of conventional optical microscopes. Using an innovative approach based on Structured Illumination Microscopy technology licensed from UCSF, the N-SIM enables detailed visualization of minute intracellular structures and their functions.