Scanning Confocal Microscope – Bring imaging to life: high-speed, high-resolution confocal imaging.
Capturing high-quality 3d confocal images at ultrahigh-speed and enhanced sensitivity with a resonant scanner and galvano scanner, Nikon’s A1R+ is a powerful tool for the imaging and visualization of intracellular dynamics and interaction.
Bring imaging to life: confocal imaging advanced to levels previously unseen.
Nikon’s groundbreaking confocal imaging system offering greater speed, higher resolution and unprecedented system flexibility.
The A1+ confocal laser microscope system is Nikon’s powerful fully-automated confocal imaging system, capable of capturing high-quality confocal images of cells and molecular events at high speed and enhanced sensitivity. Ideal for facilities with a broad range of users, the A1+ has been designed with groundbreaking new optical and electronic technology innovations to provide unprecedented system quality and flexibility. New features such as Nikon’s GaAsP multi-detector unit enables brighter, even higher resolution images than ever before.
Simple and Robust. The C2+ is designed as an essential microscopy tool for the laboratory, providing powerful and robust imaging capabilities….
Point scanning confocal microscope system offering powerful new features and vastly expanded spectral imaging capabilities.
Built on a reputation of incredible stability coupled with superior optical technologies, the C2+ with its host of functions and various analytical capabilities is the perfect tool for a new microscope, or as a new accessory to a Nikon imaging system. Now fully controlled by NIS-Elements imaging software, the system includes four channel confocal fluorescence imaging, and vastly expanded spectral capabilities with the ability to capture and unmix data acquired at any channel resolution across the entire detector bandwidth.
High-definition macro to micro confocal imaging
With high-definition wide field of view images larger than 1cm with unprecedentedly high S/N ratios, the AZ-C2+ allows for imaging of whole-mount specimens, such as embryos, in a single shot. It offers a combination of low and high magnification objective lenses, optical zoom and a confocal scanning zoom function, enabling continuous imaging from macro to micro. Moreover, the AZ-C2+ allows deep imaging of in vivowhole specimens.
Faster, deeper, sharper multiphoton confocal imaging
New multiphoton and confocal microscope system for high speed, high resolution and high sensitivity multiphoton excitation and confocal fluorescence imaging.
Nikon’s A1R MP+ multiphoton confocal microscope is a unique multiphoton imaging system featuring a fast, high resolution galvanometer scanner and an ultra-high speed resonant scanner that is capable of frame rates from 30 fps at 512 x 512 pixels to as fast as 420 fps in band scan mode. This is especially important in multiphoton microscopy because of the overlap of emission spectra of probes and autofluorescence, which is often unavoidable when using a single laser line.
Streamlined multiphoton confocal for deeper, faster multiphoton imaging.
Nikon’s A1 MP+ is a streamlined version of the unique A1R MP+ multiphoton imaging system developed for simplified, cost-effective multiphoton imaging.
Nikon’s A1 MP+ multiphoton confocal microscope is a unique multiphoton imaging system featuring a high resolution galvanometer scanner. New 4 channel non-descanned detectors with higher sensitivity, reduced dark current and broad spectral range allow for real time unmixing of closely spaced probes for accurate and high-contrast spectral imaging. This is especially important in multiphoton microscopy because of the overlap of emission spectra of probes and autofluorescence, which is often unavoidable when using a single laser line.
Enables single molecule visualization, allowing dynamic observation and functional analyses of both in vitro and living cells
Illuminator for TIRF Applications
Newly developed motorized laser TIRF illumination unit allows laser incident angle adjustment, shutter control and switching to widefield fluorescence excitation with the control pad or NIS-Elements software. The laser incident angle can be stored with a single touch of the control pad button. Stored laser incident angles can be easily reproduced. This enables alternate time-lapse recording between fluorescence and multi-wavelength TIRF images.
Modular illumination system with ultimate flexibility and expandability
The Nikon Ti-LAPP system provides modular illuminators for total internal reflection fluorescence (TIRF),photoactivation/conversion, photobleaching and epi-fluorescence.
Each module can be flexibly combined to build microscope systems that are optimized for individual research needs. For example, multiple TIRF modules can be incorporated into a single microscope for anisotropy experiments and fast, multi-angle TIRF imaging. Combined with the Ti’s stratum structure, up to five illumination modules can be incorporated into a single microscope (e.g. two TIRFs, a FRAP, a DMD and an Epi-FL module can all be integrated into one Ti).
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Ti-LAPP Modular Illumination System
All-new super-resolution microscope featuring acquisition speeds ten times faster than conventional N-STORM systems.
Nikon’s Next Generation N-STORM Super-Resolution Microscope System.
With ten times faster acquisition speeds compared to conventional N-STORM, N-STORM 4.0 enables imaging of live cells with nanoscale resolution.
Nikon has established a leading position in the field of super resolution microscopy since the release of N-STORM in 2010. STORM, or Stochastic Optical Reconstruction Microscopy is a super-resolution technique developed by Dr. Xiaowei Zhuang, a Howard Hughes Investigator at Harvard University. The technology localizes temporally isolated fluorescent molecules to reconstruct images with nanoscale resolution (~10 times greater resolution compared to conventional light microscopy). N-STORM 4.0 is the next step in the evolution of STORM imaging, enabling STORM imaging of dynamic processes in live cells.
N-STORM 4.0 utilizes an improved laser excitation design and a sCMOS camera that dramatically improves acquisition rates.
There is a rapidly growing interest in super-resolution microscopy as evidenced by the recent 2014 Nobel Prize in Chemistry awarded to the field of super-resolution microscopy. There is also an increasing need to expand these techniques to broader applications like live-cell imaging. Recent studies show that localization-based super-resolution imaging of live cells can provide unprecedented insights into the nanoscale dynamics of intracellular structures such as focal adhesions1, transferrin clusters in clathrin-coated pits2, and organelles including mitochondria and endoplasmic reticulum3.
N-STORM 4.0 utilizes an improved laser excitation design and a sCMOS camera to improve acquisition rates from minutes to seconds while gathering incredibly high molecule counts. In addition, the new system offers expanded flexibility in the imaging field of view, allowing users to choose the best suited mode for their application, whether it be high-speed imaging of live cells or gathering large fields of view of fixed cells.
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N-STORM 4.0 utilizes an improved laser excitation design and a sCMOS camera that dramatically improves acquisition rates.
1 Shroff, H. et al., 2008, Nature Methods, 5 (5), p417-423.
2 Jones, S.A. et al., 2011, Nature Methods, 8 (6), p499-505.
3 Shim, S-H. et al., 2012, Proc. Natl. Acad. Sci., 109, p13978-13983
Super-resolution microscope system which exceeds traditional diffraction limits by an order of magnitude and is capable of 3D image acquisition.
Super-Resolution microscope system offering ten times the resolution of conventional optical microscopes.
N-STORM is a super-resolution microscope system that combines “STochastic Optical Reconstruction Microscopy” technology (licensed from Harvard University) and Nikon’s Eclipse Ti research inverted microscope. The N-STORM super-resolution microscope provides dramatically enhanced resolution that is 10 times that of conventional optical microscopes.
