By capturing the intrinsic refractive index (RI) of cells using a low level of light intensity, Holotomography has emerged as a unique solution for live cell imaging that surpasses the compromise between obtaining high image quality and maintaining healthy cells.
Empowering your exploration. Redefining the limits.
The lastest generation of Holotomography – Tomocube HT-X1 – utilizes a low-coherence light source. This groundbreaking advancement unlocks label-free 3D and 4D quantitative live cell imaging on diverse multi-well plates with improved resolution and free of laser-induced speckle noise.
The new system works well with a wide range of biological samples, including confluent cell sheets and thick specimens. It provides not only high-content 3D visualization but also quantitative data for individual cells and their intracellular structures, such as the volume, surface area, and dry mass.
Multiplying Knowledge from 2D to 4D and Beyond
Observations of cellular morphology and activity are typically based on labeling of target molecules. These methods are invasive, affect the nature of the target molecules and potentially interfere with their biological relevance. In addition, these forms of label are not effective in providing quantitative information. The tools used to visualize labels are generally damaging to cells as often laser-based illumination is used to excite fluorochromes which in turn causes phototoxic damage to the cells.
The “holy grail” of imaging is to visualize cells without labeling to ensure the cells behave and grow normally. Holotomography (HT) does this by capturing the intrinsic light scattering properties of cellular materials using very low levels of light intensity, just enough to allow the light to pass through the cell. In doing so, the refractive index (RI) information of structures within the cell can be collected and selectively pseudo-colored to reveal the cell and its organelles.
In capturing the RI distribution, the Tomocube HT-X1 Holotomography can also provide quantitative data in 3D such as the volume, surface area, and dry mass of cells and their intracellular structures.
Balor is Andor’s game-changing, very large area sCMOS camera platform for Astronomy. It launches with the Balor 17-12 model, featuring an enhanced FoV 16.9 Megapixel, 70mm diagonal sensor, coupled with fast, low noise readout capability. Balor is ideal for measuring photometric and astrometric variability from milliseconds to tens of seconds timescales.
Balor is the largest commercially available sCMOS camera, designed for ‘dynamic astronomy’ applications such as Orbital Debris tracking, Solar Astronomy, Solar System Object detection, Exoplanet Discovery, Atmospheric Studies and Fast Time Resolution Astrophysics. Balor lends itself particularly well to the ‘atmospheric freezing’ techniques of Speckle/Lucky Imaging, enabling resolution enhancement of ground-based astronomy over a much larger field of view than is readily achievable through use of adaptive optics.
Nanoscribe- Quantum X shape – Tomocube HT-X1 Holotomography – Quantum X shape is a truly capable multi-talent. Based on Two-Photon Polymerization (2PP).
Fastest and most accurate 3D printer for high-end microfabrication tasks
Quantum X shape is a truly capable multi-talent. Based on Two-Photon Polymerization (2PP), the 3D laser lithography system combines proprietary 3D printing technologies that make it the optimal tool for rapid prototyping and wafer-scale batch processing of virtually any 2.5D and 3D shape with submicron precision and accuracy.
Reshaping precision.
As the second high-resolution two-photon lithography system in the industry-proven Quantum X platform, Quantum X shape offers high-resolution 3D Microfabrication capabilities with unmatched precision, next to Nanoscribe’s breakthrough technology of Two-Photon Grayscale Lithography (2GL ®) for surface patterning. The new Quantum X shape’s superior precision relies on the highest voxel modulation rate in class, and an extremely fine address grid, allowing for sub-voxel size shape control. In addition, you benefit from the 2GL voxel tuning capability for 2.5D structures with stunningly smooth, accurately shaped, or micropatterned surfaces.
Reshaping output.
Quantum X shape is the ideal additive manufacturing tool for rapid prototyping of application designs in biomedical devices, microoptics, microelectromechanical systems (MEMS), microfluidics, surface engineering and many more. Wafer handling capabilities make wafer-scale batch processing and small series production of 3D microparts easier than ever.
Reshaping usability.
Control your print job via the device’s integrated touchscreen. Keep an eye on your two-photon lithography system even from the office and in multi-user configurations via nanoConnectX. And benefit from industrial standards and time-efficient wafer batch production.
Technical features in brief
Rapid prototyping with highest precision and design freedom along a straightforward workflow
Industry-proven platform for wafer-scale batch processing
Highest resolution 3D printer with A2PL® technology for nanoprecise alignment
The Aligned 2-Photon Lithography (A2PL®) system Quantum X align enhances Nanoscribe’s field-proven 3D Microfabrication technology based on Two-Photon Polymerization (2PP) by adding high-precision alignment capabilities for highly accurate placement of printed structures. With A2PL freeform microoptics can be printed precisely aligned to the optical axes of fibers or photonic chips with submicron accuracy using this highest resolution 3D printer with nanoprecision aligned 3D printing capabilities. Produce efficient optical interconnects for photonic integration and photonics packaging or miniaturized imaging optics, e.g. for minimally invasive endoscopy.
Technical features in brief
High-performance 3D Microfabrication by Aligned 2-Photon Lithography (A2PL)
3D printing on fibers: Precisely aligned printing on the facets of optical fibers based on fiber core detection
3D printing on chips: Precisely aligned printing on the surface or facet of chips based on 3D substrate topography mapping
3D alignment: Automatic detection and compensation of substrate tilt in 3 rotation axes
The Quantum X bio is an exceptionally capable, versatile bioprinter with the highest resolution available. Powered by Two-Photon Polymerization (2PP) and grounded in engineering excellence, the proprietary technology is customized and reimagined through the eyes of a biologist.
The light-based 3D bioprinter offers essential features such as precise temperature and humidity control, HEPA-filtered airflow and an optional connection for pre-mixed air/CO2. A variety of functionalized biomaterials can be used to unlock a new level of bioprinting and effectively accelerate innovation in tissue engineering, microbiology, materials engineering and biomedical devices.
Bring your ideas to life with the highest precision 3D printing
Confocal microscopes have been commercially available now for over 25 years. How can newer iterations of a fundamentally simple instrument continue to innovate? What changes can redefine how a confocal is used, and what data can be collected? Introducing the Nikon AX/AX R Confocal Microscope System, our 10th generation point scanning confocal, giving you more of everything: Leveraging Artificial Intelligence (AI), expanding the number of colors, improving pixel density, sensitivity and speed.
These are significant additions in terms of expanding the range of experiments possible with a point scanning confocal, while increasing the usability and functionality of the instrument, all in a modular and upgradable platform.
Nikon AX is the new standard in confocal imaging.
Igniting New Confocal Imaging Potential
The newly developed Nikon Spatial Array Confocal (NSPARC) detector utilizes an ultra-low noise detector array to collect a two-dimensional image at each scanned point. This method of image scanning microscopy (ISM) improves signal-to-noise ratio by increasing the available signal level while simultaneously allowing imaging with lower excitation power.
Single-photon sensitivity and array detection extend the capabilities of the AX system by revealing unseen details in every image, while array detection pushes the boundaries of resolution beyond the theoretical limits.
Luminosa pairs highest data quality with remarkably simple day-to-day operation. It easily integrates into any researcher’s toolbox and becomes a time-efficient, reliable companion for scientists starting to explore the use of time-resolved fluorescence methodologies as well as for experts wanting to push the limits. Truly a microscopy system that everybody can rely on.
PicoQuant Fluorescence Lifetime Spectrometer – FluoTime 250 integrates all essential optics and electronics for time-resolved luminescence spectroscopy in a compact, fully automated device.
Compact and Modular Fluorescence Lifetime Spectrometer
Fully automated compact and modular system
Filter based emission selection, optional monochromator
Supports both TCSPC and Multi-Channel Scaling data acquisition
Step by step application wizards and scripting option
Covers lifetime ranges from picoseconds to milliseconds
A compact spectrometer for routine and complex measurements
PicoQuant Fluorescence Lifetime Spectrometer – FluoTime 250 integrates all essential optics and electronics for time-resolved luminescence spectroscopy in a compact, fully automated device. This spectrometer is designed to assist the user in carrying out routine as well as complex measurements easily and with high reliability thanks to fully automated hardware components and a versatile system software featuring wizards providing step-by-step guidance.
Fully automated with high performance optics
All components (attenuators, polarizers and filter wheel) in the optical beam paths are motorized and controlled by the EasyTau 2 software. Emission wavelength selection is implemented via a motorized filter wheel equipped with various cut-off or bandpass filters as selected by the customer. An optional monochromator is available for the UV/VIS spectral range.
Flexible excitation sources
The FluoTime 250 uses picosecond pulsed lasers diodes and LEDs from PicoQuant’s LDH and PLS series, covering a spectral range from 255 to 1550 nm. All excitation sources are controlled via a PDL 820 laser driver, which allows not only to vary output power and repetition rates (up to 80 MHz) but also supports burst mode for selected laser heads.
TCSPC and MCS based data acquisition
Several outstanding data acquisition units are available with various temporal resolutions (from 4 ps to 250 ns). Coupled with the right detectors from the PMA or PMA Hybrid Series, the FluoTime 250 is capable of measuring time-resolved emissions with lifetimes ranging from approximately 10 ps up to several hundreds ms.
A choice of detectors
The FluoTime 250 can be equipped with a single photon counting detector from either the PMA or PMA Hybrid Series.These detectors offer picosecond temporal resolutions and cover different spectral ranges between 180 and 920 nm. For studies in the near infrared, a PMT module with sensitivity from 950 to 1400 nm is also available. Each detector includes an electro-mechanical shutter, active cooling, and overload protection.
System software EasyTau 2
The EasyTau 2 software package is the one-stop solution for both full hardware control of the FluoTime 250 as well as for interactive data analysis and fitting. The software offers a Windows-based graphical user interface with dedicated applications wizards guiding users through the optimization and data acquisition process for many common applications. Advanced users get full control over all instrumental aspects thanks to a customized mode and scripting language. The integrated analysis and fitting module supports a broad range of time-resolved spectroscopy applications, such as fluorescence and phosphorescence decays, or anisotropy measurements. A powerful report generator allows creating presentation-ready graphical and numerical output.
